Identification of oral bacteria by 16S rRNA gene analysis in elderly persons requiring nursing care |
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Authors: | Hirotaka Kurabayashi Akihiro Kaneko Ryo Sekiya Kazunari Karakida Masashi Sasaki Noriko Nakatogawa Takayuki Aoki Yoshihide Ota Haruo Sakamoto |
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Institution: | (1) Department of Oral Surgery, Tokai University, School of Medicine, 143 Shimokasuya, Isehara Kanagawa, 259-1193, Japan; |
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Abstract: | After incubation of saliva from 58 semi-bedridden elderly persons, the cultures were identified based on the 16S rRNA gene
base sequence to compare the identification by the conventional culture method. As a result, the 16S rRNA gene base sequence
of 198 strains identified by the culture method showed 98.5% or more homology in some of the Human Oral Microbiome database,
and the identification of bacterial species and genus was possible. When an organism identified by the 16S rRNA gene sequencing
method was compared with that by the culture method, the concordance rates were 54.5% at the genus level and 35.9% at the
species level. Streptococcus mitis strains most frequently isolated from saliva that were identified by the culture method were identified as the same species
by the 16S rRNA gene sequencing method (32/35), and all the 11 Streptococcus salivarius strains identified by the culture method were identified as the same species by the 16S rRNA gene sequencing method. All
the strains identified as Streptococcus anginosus group by the culture method and 8 of the 9 strains identified as Prevotella species by the culture method were identified as the same group and genus by the 16S rRNA gene sequencing method. When an
oral microbial flora test with saliva samples from elderly persons is performed, the 16S rRNA gene sequence identification
enables us to identify major indigenous bacteria and pathogenic bacteria and is considered useful as a means of supplementing
the conventional culture method. |
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Keywords: | 16S rRNA gene Identification Saliva Oral cavity Microbial flora |
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