HCCR-1、P53在宫颈癌及其癌前病变中的表达及其临床意义

目的]探讨人宫颈癌致癌基因-1（human cervical cancer oncogene-1，HCCR-1）、P53在宫颈癌及其癌前病变组织中的表达及临床意义。比较人乳头状瘤病毒（human papillomavirus ，HPV）检测与免疫组化法检测HCCR-1蛋白诊断宫颈上皮内瘤样变（cervical intraepithelianeoplasia ，CIN ）和宫颈癌的敏感度和特异度。方法]采用免疫组化SP法检测 HCCR-1和P53在具有 HPV-DNA检测结果的40例宫颈癌、30例CIN Ⅰ、30例CINⅡ～Ⅲ和10例正常宫颈组织标本中的表达。结果]① HCCR-1蛋白在正常宫颈组织中无表达，随着宫颈上皮病变的升级而呈现增高的趋势，各组间差异有统计学意义（ P ＜0．05）。②CIN Ⅰ、CIN Ⅱ～Ⅲ和宫颈癌P53表达阳性率显著高于正常宫颈（ P ＜0．05）。③ HCCR-1、P53在宫颈组织中的表达 HCCR-1、P53在宫颈组织的表达相关系数较低（ r ＝0．220，P ＝0．021），可认为两者没有相关性。④ HCCR-1、HR-HPV在宫颈组织的表达明显呈正相关（ r ＝0．9，P ＝0．000）。⑤免疫组化法检测 HCCR-1诊断CIN和宫颈癌的敏感度和特异度分别为71％和100％。结论]在宫颈病变进展的过程中受多基因调控。 HCCR-1与突变型p53蛋白的表达随病理级别进展均呈逐步增加的趋势，在宫颈病变的发生发展中可能起着重要的作用。免疫组化法检测宫颈组织中 HCCR-1蛋白表达有望成为宫颈病变的诊断辅助工具。

Expressions of HCCR-1 and p53 in Cervical Cancer and Precancerous Lesion and Their Significance

Objective]To explore the expressions and clinical significance of human cervical cancer oncogene-1（HC-CR-1） and p53 in uterine cervix carcinoma（UCC）and cervical lesion .The sensitivity and specificity between HPV DNA test and immunohistochemical detection of HCCR-1 for the diagnosis of CIN and UCC were compared .Methods]Immu-nohistochemical staining S-P method was used to detect the expressions of HCCR-1 and p53 in 40 cases of UCC ,30 cases of cervical intraepithelial neoplasia （CIN）Ⅰ,30 cases of CIN Ⅱ~ Ⅲ and 10 cases of normal cervix tissues with the results by HPV-DNA .Results]There was no positive expression of HCCR-1 in normal cervix tissues .The expression of HC-CR-1 was increased with the degree of cervical lesion ,and there were significant differences among groups （ P 〈0 .05） . The coefficient correlation between HCCR-1 and p53 in cervix tissues was lower（ r=0 .220 ,P=0 .021） or there was no correlation between them .There was obviously positive correlation between HCCR-1 and HR-HPV in cervix tissues（ r=0 .9 ,P=0 .000） .The sensitivity and specificity of immunohistochemical detection of HCCR-1 for the diagnosis of CIN and UCC were 71% and 100% ,respectively .Conclusion]Multiple gene regulation involves in the development of cervi-cal lesion .The expressions of HCCR-1 and mutant p53 are gradually increased with the grade of lesion ,and play an im-portant role in the pathogenesis and development .Immunochemical method for the detection of HCCR-1 protein in cervix tissues may be used as the assistant means for the diagnosis of cervical lesion .