| Institution: | 1. Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;2. Morphological Laboratory, Expeimental Research Center, Research and Development Service Base, China Academy of Chinese Medical Sciences, Beijing 100700, China;3. First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China |
| Abstract: | ObjectiveTo investigate the neural differentiation capacity of water extraction of velvet antler.MethodsVelvet antler (Cervus Nippon Temminck) polypeptide (VAP) was used to differentiate neural stem cells (NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immunofluorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.ResultsThe differentiating cells look like neuron, some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron -like cells can express the special neural molecules.ConclusionDifferentiation of NSCs towards neurons can be induced by velvet antler polypeptide. |
