Adenovirus type 12 tumor antigen. II. Immunoprecipitation of protein kinase from infected and transformed cells by antisera to T antigen and some normal rat sera. |
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Authors: | Karel Raška Arnold Geis Bernd Föhring |
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Affiliation: | Department of Pathology, College of Medicine and Dentistry of New Jersey-Rutgers Medical School, Piscataway, New Jersey 08854, USA |
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Abstract: | Protein kinase was found to be precipitated from adenovirus type 12 (Ad12)-infected KB cells and Ad12-transformed hamster cells by sera of tumor-bearing hamsters and rats: Immunoprecipitates obtained with T antigen reactive sera catalyzed transfer of 32P from [γ-32P]ATP to the γ-chain of IgG. Analogous products of control cells were without significant activity. Control hamster sera precipitated no protein kinase from infected and transformed cells. Some control rat sera (syngeneic with immune sera), however, were found to precipitate protein kinase from infected and transformed cells; particularly active in this respect were sera of female breeder rats. When partially purified, highly immunoreactive T antigen preparations from transformed cells were used as a source of enzymatic activity, protein kinase was detected only in precipitates obtained with immune sera. |
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Keywords: | To whom reprint requests should be addressed. |
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