| 双缩脲法和溴甲酚绿法测定胸腹水总蛋白和白蛋白的检测范围评价 |
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| 引用本文： | 陈筱菲,徐来. 双缩脲法和溴甲酚绿法测定胸腹水总蛋白和白蛋白的检测范围评价[J]. 中国卫生检验杂志, 2011, 0(1) |
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| 作者姓名： | 陈筱菲 徐来 |
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| 作者单位： | 温州医学院第一附属医院临床实验诊断中心;温州医学院; |
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| 摘 要： | 目的:确定双缩脲法测定胸腹水总蛋白和溴甲酚绿法测定胸腹水白蛋白的检测限,并做其检测范围线性评价。方法:制备空白样品,选择近检测限和病理高值的病人样品。在日立7600全自动生化分析仪上分别用双缩脲法测定总蛋白和溴甲酚绿法测定白蛋白,做检测低限和生物检测限分析,采用EP-6P做线性评价和多项式做线性评价。结果:双缩脲法测定胸腹水总蛋白的检测低限为0.47 g/L,生物检测限为1.33 g/L,2.5 g/L~49.7 g/L范围内线性符合临床要求。溴甲酚绿法测定白蛋白的检测低限为0.44 g/L,生物检测限为0.98 g/L,1.2 g/L~35.2 g/L范围内线性符合临床要求。结论:双缩脲法测定胸腹水总蛋白和溴甲酚绿法测定胸腹水白蛋白能满足临床要求。
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| 关 键 词： | 胸腹水 总蛋白 白蛋白 检测限 检测范围 |
Evaluation of the range of detection of determining hydrothorax and ascites total protein with biuret and albumin with bromocresol green method |
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| CHEN Xiao-fei,Xu Lai. Evaluation of the range of detection of determining hydrothorax and ascites total protein with biuret and albumin with bromocresol green method[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(1) |
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| Authors: | CHEN Xiao-fei Xu Lai |
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| Affiliation: | CHEN Xiao-fei1,Xu Lai2(1.Clinical Laboratory Diagnositc Center,Wenzhou Medical College First Affiliated Hospital,Wenzhou,Zhejiang Province,325000,China,2.Internship Student,Wenzhou Medical College) |
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| Abstract: | Objective:Determine the detection limit of hydrothorax and ascites total protein determining with biuret,as well as hydrothorax and ascites albumin determining with bromocresol green method,and perform linearity evaluation of the range of detection.Methods: Prepare blank sample,select samples that are close to the detection limit and those that have high pathology value.Perform total protein determining with biuret,and albumin determining with bromocresol green method on Hitachi 7600 Automatic Biochemic Ana... |
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| Keywords: | Hydrothorax and Ascites Total Protein Albumin Limit of Detection Range of Detection |
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