| 基于免疫分选的T淋巴细胞亚群嵌合性定量分析 |
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| 引用本文： | 许先国,章伟,陈舒,朱发明,吕杭军,严力行. 基于免疫分选的T淋巴细胞亚群嵌合性定量分析[J]. 中国卫生检验杂志, 2011, 0(1): 101-102,105 |
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| 作者姓名： | 许先国 章伟 陈舒 朱发明 吕杭军 严力行 |
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| 作者单位： | 浙江省血液中心输血研究所卫生部血液安全研究重点实验室; |
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| 基金项目： | 浙江省医卫科研基金(2008A036); 浙江省医卫青年人才基金(2008QN008) |
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| 摘 要： | 目的:建立基于免疫分选的T淋巴细胞亚群嵌合性定量分析的方法。方法:以淋巴细胞不同比例混合制备14组人工嵌合体血样,以免疫磁珠阳性选择法获取CD4+和CD8+T细胞亚群,分选后的细胞再进行16位点的短串重复序列(STR)多态性分析。结果:分选后T细胞亚群提取的DNA量能满足STR检测要求,当嵌合体中受体比例≥10%时,16个STR位点均能检出受体和供体特异性等位基因,按照STR峰面积计算的受体比例与理论值相符;当受体比例≤1%时,仅有部分位点能检出受体特异性等位基因,实测嵌合率与理论值存在差异。结论:建立了基于免疫分选的T细胞亚群嵌合性定量分析方法,可用于造血干细胞移植术后嵌合体监测。
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| 关 键 词： | 嵌合体 免疫分选 造血干细胞移植 |
Quantitative chimerism analysis of T lymphocyte subsets based on immune sorting |
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| XU Xian-Guo,ZHANG Wei,CHEN Su,ZHU Fa-Ming,LV Hang-jun,YAN Li-Xing. Quantitative chimerism analysis of T lymphocyte subsets based on immune sorting[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(1): 101-102,105 |
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| Authors: | XU Xian-Guo ZHANG Wei CHEN Su ZHU Fa-Ming LV Hang-jun YAN Li-Xing |
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| Affiliation: | XU Xian-Guo,ZHANG Wei,CHEN Su,ZHU Fa-Ming,LV Hang-jun,YAN Li-Xing(Blood Center of Zhejiang Province,Key Laboratory of Blood Safety Research,MOH,Hangzhou 310006,China) |
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| Abstract: | Objective:To establish a quantitative method for chimerism analysis of T lymphocyte subsets based on immune sorting.Methods: 14 artificial chimera groups were mixed according to different lymphocytes proportion.The CD4+ and CD8+ T cell subsets were sorted by positive selection of immunomagnetic beads,and then were analyzed by STR polymorphisms technique.Results: The DNAs extracted from sorted T cells were fit for STR detection.All STR alleles specific for recipient or donor can be detected and the quantitat... |
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| Keywords: | Chimera Immune sorting Hematopoietic stem cell transplantation |
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