| 反式BPDE诱发的转化细胞与成瘤细胞FHIT基因变化研究 |
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| 引用本文： | 黄勇,陈家堃,吴中亮,吕嘉春,纪卫东,蒋义国. 反式BPDE诱发的转化细胞与成瘤细胞FHIT基因变化研究[J]. 中国卫生检验杂志, 2011, 0(1) |
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| 作者姓名： | 黄勇 陈家堃 吴中亮 吕嘉春 纪卫东 蒋义国 |
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| 作者单位： | 广东省东莞市疾病预防控制中心;广州医学院化学致癌研究所; |
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| 摘 要： | 目的:探讨苯并(a)芘[B(a)P]代谢产物反式二氢二醇环氧苯并芘[anti-7,8,-dihydrodiol-9,10-epoxidebenzo(a)pyrene,BPDE]引起的细胞FHIT基因突变情况。方法:PCR、RT-PCR、多聚酶链聚合反应-单链构象多态性分析(PCR-SSCP)。结果:PCR-SSCP结果提示反式BPDE诱发的恶性转化细胞及其裸鼠成瘤细胞FHIT基因的Exon1-9出现了异常的小片断。结论:反式BPDE可作用于细胞染色体3p14.2的脆性部位FRA3B,引起FHIT基因的重排或缺失,导致FHIT基因功能失活。推测FHIT基因可能是反式BPDE致癌作用的靶分子之一。
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| 关 键 词： | 苯并(a)芘[B(a)P] 反式BPDE 人支气管上皮细胞 FHIT基因 |
The alterations of FHIT gene in malignant transformation cells of human bronchial epithelial cells induced by anti-BPDE |
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| HUANG Yong,CHEN Jia-kun,WU Zhong-liang,LU Jia-chun,JI Wei-dong,JIANG Yi-guo. The alterations of FHIT gene in malignant transformation cells of human bronchial epithelial cells induced by anti-BPDE[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(1) |
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| Authors: | HUANG Yong CHEN Jia-kun WU Zhong-liang LU Jia-chun JI Wei-dong JIANG Yi-guo |
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| Affiliation: | HUANG Yong1,CHEN Jia-kun2,WU Zhong-liang2,LU Jia-chun2,JI Wei-dong2,JIANG Yi-guo2(1.The Center for Disease Control and Prevention of Dongguan City,Dongguan 523006,China,2.The Institute for Chemical Carcinogenesis of Guangzhou Medical College,Guangzhou 510182,China) |
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| Abstract: | Objective:To research the alterations of FHIT gene in malignant transformation cells of human bronchial epithelial cells caused by the metabolism of Benzo(a)pyrene anti-7,8,-dihydrodiol-9,10-epoxide benzo(a)pyrene(BPDE).Methods: PCR,RT-PCR and PCR-SSCP(Single strand confirmation polymorphism,SSCP).Results: Compared with the control cells,the high dose administration transformed cells and tumor cells present some short fragment of the FHIT gene Exon1-9,the length of the FHIT gene Exon1-9 of low dose administ... |
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| Keywords: | Benzo(a)pyrene BPDE Human brochial epithelial cell FHIT gene |
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