| 食品及环境阪崎肠杆菌分离株的MLST和PFGE分型方法比较 |
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| 引用本文： | 李秀娟,赵冬,田会方,徐保红,钟炜.食品及环境阪崎肠杆菌分离株的MLST和PFGE分型方法比较[J].中国卫生检验杂志,2011(3). |
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| 作者姓名： | 李秀娟 赵冬 田会方 徐保红 钟炜 |
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| 作者单位： | 河北省石家庄市疾病预防控制中心;河北省石家庄市裕华区妇幼保健站; |
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| 摘 要： | 目的:对PFGE和MLST方法在阪崎肠杆菌分型研究中的分辨力和潜在价值进行了比较研究和论述。方法:采用PFGE和MLST方法对本实验室分离的19株阪崎肠杆菌和一株标准菌株ATCC51329进行分型研究。结果:PFGE方法可将20株阪崎肠杆菌分为6大类群,共16个PFGE型,分辨力为0.9474。而MLST方法可将20株菌株分为12个ST型,分辨力为0.9263。结论:PFGE法较MLST法具有较高的分辨力,可用于阪崎肠杆菌的溯源研究,而MLST分型方法能通过全球比对数据库得到更多的关于进化和亲缘关系的分析资料,在致病研究、流行病学调查、进化研究方面优于PFGE法。
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| 关 键 词： | 阪崎肠杆菌 多位点序列分型(MLST) 脉冲场凝胶电泳(PFGE) |
Comparative analysis of multilocus sequence typing and pulse-field gel electrophoresis in typing of Enterobacter sakazakii isolated from foods and environment |
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| LI Xiu-juan,ZHAO Dong,TIAN Hui-fang,XU Bao-hong,ZHONG Wei.Comparative analysis of multilocus sequence typing and pulse-field gel electrophoresis in typing of Enterobacter sakazakii isolated from foods and environment[J].Chinses Journal of Health Laboratory Technology,2011(3). |
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| Authors: | LI Xiu-juan ZHAO Dong TIAN Hui-fang XU Bao-hong ZHONG Wei |
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| Institution: | LI Xiu-juan1,ZHAO Dong1,TIAN Hui-fang1,XU Bao-hong1,ZHONG Wei2 (1.Shijiazhuang Center for Disease Control and Prevention,Shijiazhuang 050011,China,2.Yuhua District,Shijiazhuang,Maternal and Child Health Station,China) |
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| Abstract: | Objective:To compare discriminatory ability and potential value of PFGE and MLST which were used as a typing method of Enterobacter sakazakii.Methods:19 strains Enterobacter sakazakii isolates and a standard strain ATCC51329 were typed by PFGE and MLST separately.Results:20 stains Enterobacter sakazakii were divided into 6 clusters including 16 PFGE types with Discriminatory index 0.9474 while they were divided into 12 ST types by MLST with Discriminatory index 0.9263.Conclusion:PFGE had higher discriminato... |
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| Keywords: | Enterobacter sakazakii Multilocus sequence typing Pulse-field gel electro phoresis |
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