| 基于可溶性虫卵抗原的血吸虫抗体检测免疫传感器 |
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| 引用本文： | 马静,周卓晟,徐勇,刘红艳,余枫华,刘军,杨春秀. 基于可溶性虫卵抗原的血吸虫抗体检测免疫传感器[J]. 中国卫生检验杂志, 2011, 0(1) |
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| 作者姓名： | 马静 周卓晟 徐勇 刘红艳 余枫华 刘军 杨春秀 |
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| 作者单位： | 湖北省宜昌市疾病预防控制中心;华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室; |
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| 基金项目： | 宜昌市2009年医疗卫生科技攻关项目(A09310-42) |
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| 摘 要： | 目的:将免疫法特异性和电化学法灵敏性相结合,建立一种高灵敏基于可溶性虫卵抗原的电化学免疫传感器,用于血吸虫抗体的检测。方法:巯基乙胺通过吸附作用在金电极表面形成的自组装单分子层(SAM),依次滴加2.5%戊二醛和血吸虫抗原(可溶性虫卵抗原,soluble egg antigen,SEA),用以捕获抗SEA抗体(兔多抗或人多抗),然后再与辣根过氧化物酶(HRP)标记的二抗形成免疫复合物。用循环伏安法检测还原峰的电流值。结果:在底物为添加了2μl 30%的双氧水及1.0 mmol/L对苯二酚的0.1 mol/L pH7.2的PBS缓冲液中,还原峰的电流值与抗SEA兔血清稀释度在10-3与10-6范围内成正比,相关系数为0.956。与抗SEA人血清稀释比在10-1与10-4范围内成正比,相关系数为0.988。在1∶40稀释度条件下,血吸虫病人与正常人血清的抗SEA还原峰的电流值相差7.4μA。结论:该方法可以用于血吸虫抗体的初步检测。
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| 关 键 词： | 血吸虫抗原 SEA 酶免疫传感器 循环伏安 |
The soluble egg antigen-based immunosensor for detection of Schistosome antibodies |
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| MA Jing,ZHOU Zhuo-cheng,XU Yong,LIU Hong-yan,YU Feng-hua,LU Ju,YANG Chun-xiu. The soluble egg antigen-based immunosensor for detection of Schistosome antibodies[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(1) |
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| Authors: | MA Jing ZHOU Zhuo-cheng XU Yong LIU Hong-yan YU Feng-hua LU Ju YANG Chun-xiu |
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| Affiliation: | MA Jing1,ZHOU Zhuo-cheng2,XU Yong1,LIU Hong-yan2,YU Feng-hua1,LU Ju1,YANG Chun-xiu(1.Centre for Disease Control and Prevention of city YiChang,YiChang 443000,China,2.MOE Key Lab of Environment and Health,School of Public Health,Tongji Medical Colege,Huazhong University of Science & Technology,Wuhan 430030,China) |
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| Abstract: | Objective:Through the combination of specific immunoassay and sensitive electrochemical method,a highly sensitive electrochemical immunosensor for Schistosoma antibodies was established,which was based on the soluble egg antigen(SEA) of Schistosome.Methods: The self-assembled monolayer(SAM) was formed by mercaptoethylamine adsorbing on gold electrode surface,folowed the dropping of 2.5% glutaraldehyde and SEA to capture Schistosoma antibodies(rabbit or human polyclonal antibodies).Then the immune complex fo... |
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| Keywords: | Schistosoma antigen SEA Enzyme immunoassay sensor Cyclic voltammetry |
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