| 白细胞介素24对大鼠脑胶质瘤细胞Survivin表达的影响 |
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| 引用本文： | 刘英姿,张磊,单保恩,张学新.白细胞介素24对大鼠脑胶质瘤细胞Survivin表达的影响[J].中国微侵袭神经外科杂志,2011,16(4):177-179. |
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| 作者姓名： | 刘英姿 张磊 单保恩 张学新 |
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| 作者单位： | 1. 河北医科大学第四医院神经外科,石家庄,050012
2. 河北医科大学第四医院科研中心,石家庄,050012 |
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| 基金项目： | 河北省科技厅2006年指导性计划项目(编号:062761308) |
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| 摘 要： | 目的探讨白细胞介素24(IL-24)基因对荷瘤大鼠脑胶质瘤细胞Survivin表达的影响。方法 48只SD大鼠随机分为实验组和对照组,每组24只,分别接种IL-24/C6细胞(转染IL-24基因的C6鼠脑胶质瘤细胞)和C6鼠脑胶质瘤细胞。接种21 d后,采用RT-PCR、Western blot和免疫组化方法检测两组大鼠脑肿瘤组织中Survivin mRNA和蛋白的表达,采用流式细胞学检测肿瘤细胞凋亡率。结果 RT-PCR检测显示:实验组大鼠脑肿瘤组织中Survivin mRNA表达水平明显低于对照组(P<0.01)。Western blot及免疫组化法检测显示:实验组大鼠脑肿瘤组织中Survivin的蛋白水平明显低于对照组(P<0.01)。流式细胞学结果显示:实验组细胞凋亡率显著高于对照组(P<0.01)。结论 IL-24可抑制大鼠胶质瘤中Survivin mRNA和蛋白的表达,促进细胞凋亡,抑制肿瘤生长。
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| 关 键 词： | 神经胶质瘤 白细胞介素24 Survivin蛋白 Survivin mRNA 大鼠 |
Effect of interleukin-24 on expression of Survivin in glioma cells |
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| Liu Yingzi,Zhang Lei,Shan Baoen,Zhang Xuexin.Effect of interleukin-24 on expression of Survivin in glioma cells[J].Chinese Journal of Minimally Invasive Neurosurgery,2011,16(4):177-179. |
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| Authors: | Liu Yingzi Zhang Lei Shan Baoen Zhang Xuexin |
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| Institution: | Liu Yingzi1,Zhang Lei1,Shan Baoen2,Zhang Xuexin1 1.Department of Neurosurgery,2.Centre of Scientific Research,The Fourth Hospital,Hebei Medical University,Shijiazhuang,Hebei 050011,China |
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| Abstract: | Objective To investigate the effect of interleukin-24(IL-24) gene on expression of Survivin in rat glioma cells.Methods Forty-eight SD rats were randomly divided into experimental group and control group,24 each.The rats in experimental group were inoculated with the rat glioma C6 cells transfected with IL-24 gene,while the rats in control group were inoculated with the rat glioma C6 cells.The brain tumor tissues in two groups were harvested 21 d after injection and apoptosis rate of gliomas cells was detec... |
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| Keywords: | glioma interleukin-24 Survivin protein Survivin mRNA rats |
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