| 人参皂苷Rg3对人肺腺癌细胞株A549/DDP抑制转移及逆转耐药作用的研究 |
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| 引用本文： | 王艳,刘静蕾,刘莉,赵红丽,李欣,杨茂鹏. 人参皂苷Rg3对人肺腺癌细胞株A549/DDP抑制转移及逆转耐药作用的研究[J]. 中国卫生检验杂志, 2011, 0(3) |
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| 作者姓名： | 王艳 刘静蕾 刘莉 赵红丽 李欣 杨茂鹏 |
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| 作者单位： | 黑龙江中医药大学; |
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| 基金项目： | 黑龙江省自然科学基金资助课题(D200849) |
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| 摘 要： | 目的:探讨人参皂苷Rg3抑制人肺腺癌细胞株A549/DDP的浸润及转移和逆转其耐药作用及机制。方法:应用MTT法检测Rg3对A549/DDP细胞逆转耐药的作用;用细胞膜流动性实验检测Rg3对A549/DDP细胞膜流动性的改变用穿膜实验检测Rg3对A549/DDP细胞侵袭和转移的影响;用Western blotting实验检测Rg3对转移和耐药相关蛋白表达的影响。结果:Rg3作用后,A549/DDP细胞对顺铂的耐药性下降I,C50明显降低(P<0.05),细胞膜的流动性明显降低(P<0.05),细胞的穿膜能力明显降低(P<0.05),Rg3作用后,nm23及caspase-3的表达明显上调,LRP的表达无明显改变。结论:Rg3可通过上调nm23的表达抑制A549/DDP细胞的侵袭和转移能力,还可通过上调caspas-3的表达,降低细胞膜的流动性达到逆转耐药的作用。
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| 关 键 词： | 人参皂苷Rg3 转移 多药耐药 |
Studies of 20(R)-ginsenoside Rg3 on restraining metastasis and reversing multidrug resistance in A549/DDP cell line |
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| WANG Yan,LIU Jing-lei,LIU Li,ZHAO Hong-li,LI Xin,YANG Mao-peng. Studies of 20(R)-ginsenoside Rg3 on restraining metastasis and reversing multidrug resistance in A549/DDP cell line[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(3) |
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| Authors: | WANG Yan LIU Jing-lei LIU Li ZHAO Hong-li LI Xin YANG Mao-peng |
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| Affiliation: | WANG Yan,LIU Jing-lei,LIU Li,ZHAO Hong-li,LI Xin,YANG Mao-peng (Heilongjiang University of Chinese Medicine,Harbin 150040,China) |
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| Abstract: | Objective:To observe and study the mechanism of restraining the metastasis and reversing multidrug resistance(MDR) of A549/DDP cell line by 20(R)-ginsenoside Rg3.Methods:MTT was applied to determine the chemosensitivity of A549/DDP to cisplatin,cell membrane lipid fluidity was determined to observe the Rg3 effect to cell,cell invasion assay was applied to determine the invasion change of Rg3 to cell,the protein expression levels of nm23,caspase-3 and LRP were determined by Western blotting analyses.Results:... |
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| Keywords: | 20(R)-ginsenoside Rg3 Metastasis Multidrug resistance |
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