无肝硬化肝癌和宿主肝甲胎蛋白免疫酶标记定位及其病理形态学研究

本文报告32例无肝硬化肝癌(NCH)的手术切除病例,并以肝硬化肝癌(CH)12例作对照,均采用常规固定作AFP免疫酶法,其结果:(1)NCH组宿主肝AFP阳性率略高于癌组织。AFP阳性物质在肝细胞内常全浆分布。以数灶性分布。3例宿主肝内增生胆管上皮AFP阳性。与CH与组比较:AFP阳性物质在细胞内,组织内的分布形式近似,不同的是宿主肝AFP阳性率和阳性强度均高于NCH组;(2)NCH组有乙型肝炎的宿主肝AFP阳性率53.13％,要显著高于地衣红阴性肝炎28.13％。(3)AFP在癌细胞内及癌组织的分布形式与宿主肝近似。AFP阳性细胞一般散在于癌组织内,但在癌梁边较多;(4)采用常规固定作AFP免疫酶法进行回预性研究是可行的。

PATHOLOGICAL OBSERVATIONS ON THE ENZYME-LABELING IMMUNO-FETOPROTEIN IN HUMAN NONCIRRHOTIC LIVER CANCER AND PERI-CANCER TISSUE

pathological observations on 32 surgical cases of noncirrhotic hepatocellular carcin-oma(NCH)were made. 12 surgical cases of cirrhotic hepatocellular carcinoma(CH) were taken as control. Routine fixed method were made by immunoperoxidase staining for AFP.The results were as follows. (1)The positive rate of NCH group host liver is somewhat higher than those of cancer tissue.AFP positive meterials were also observated in 3 cases with proliferative bile-duct epithelium, as compared with CH group,the AFP positive materials distributed in the tissue are similar to those in she cells.The differences of the AFP intensity and the positive rate of NCH group host liver are less than that of CH group.(2)In NCH group, the rate of positive cases of hepatis B is 53.13 %.it is markedly higher than that in 28.13% of orcein negative hepatitiis. (3)The AFP distributing in the cancer tissue and cancer cells were similar to those in the host liver tissue.but much more distributing in the cancer peri-trabecula.(4)It suggested that the review study by routine fixztion and staining immunoperxidase method for AFP is available progressed.