| 4-磺酸酯-2,2,6,6-四甲基哌啶氮氧自由基对大鼠组织和红细胞的抗脂质过氧化作用 |
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| 引用本文： | 葛斌,陈立德,张振明.4-磺酸酯-2,2,6,6-四甲基哌啶氮氧自由基对大鼠组织和红细胞的抗脂质过氧化作用[J].中国药理学与毒理学杂志,2001,15(1). |
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| 作者姓名： | 葛斌 陈立德 张振明 |
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| 作者单位： | 甘肃省人民医院药剂科， 甘肃 兰州 730000 |
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| 摘 要： | 研究了 4 磺酸酯 2 ,2 ,6,6 四甲基哌啶氮氧自由基 (STMP)的抗脂质过氧化作用 .结果表明 ,STMP显著抑制丙二醛 (MDA)的生成 ,其抑制心 ,肝 ,肾MDA生成的IC50 分别为 4 .4 ,3.2及 3.7μmol·L- 1;对抗溶血反应 ,其IC50 为 318.3μmol·L- 1;但不影响O2 ÷ 的生成 .提示STMP通过清除·OH而对抗脂质过氧化
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| 关 键 词： | 4-磺酸酯-2 2 6 6-四甲基哌啶氮氧自由基 自由基 丙二醛 中性白细胞 红细胞 |
Antioxidative activity of 4-sulfonate-2,2,6,6-tetramethylpiperidi- neoxyl in tissues and erythrocytes from rats |
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| GE Bin,CHEN Li De,ZHANG Zhen Ming.Antioxidative activity of 4-sulfonate-2,2,6,6-tetramethylpiperidi- neoxyl in tissues and erythrocytes from rats[J].Chinese Journal of Pharmacology and Toxicology,2001,15(1). |
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| Authors: | GE Bin CHEN Li De ZHANG Zhen Ming |
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| Abstract: | The effect of antioxidation of4-sulfonate-2,2,6,6-tetramethylpiperidineoxyl (STMP) in tissues and erythrocytes from rats were studied by determining malondialdehyde (MDA) formation induced by .OH free radical generation system (Fe2+-ascorbic acid) in homogenate of heart, liver and kidneys of rats using thiobarbituric acid colorimetric method, by measuring spectrometrically H2O2-caused hemolysis and by assaying superoxide anion from zymosan-stimulated neutrophils of rats. The results showed that STMP markedly inhibited MDA generation caused by .OH generation system with IC50 value 4.4(in heart), 3.2(in liver) and 3.7(in kidneys) μmol.L-1, respectively, antagonized H2O2-caused hemolysis (IC50=318 μmol.L-1), but did not affect O2÷ formation from activated neutrophils. It is suggested that STMP exert their antilipoperoxidative effect by specifically scavenging .OH free radicals and H2O2 in biological system. |
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| Keywords: | sulfonate 2 2 6 6 tetramethylpiperidineoxyl free radicals malondialdehyde neutrophils erythrocytes |
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