|
|||||
|
|
Measurement of human growth hormone (hGH) using a rapid immunochemiluminometric assay |
|
| Authors: | I Weeks J S Woodhead |
| Affiliation: | 1. Université de Rouen, Groupe de Physique des Matériaux, UMR 6634 CNRS, UFR Sciences et Techniques, Avenue de l''Université, BP12, 76801 Saint Etienne du Rouvray, France;2. ArcelorMittal research SA, voie romaine, 57 280 Maizières Les Metz, France;3. Université de Lorraine, Laboratoire d''étude des microstructures et de mécanique des matériaux, UMR CNRS 7239, Ile du Saulcy, 57045 Metz cedex 01, France;4. Institut de Chimie de la Matière Condensée de Bordeaux, UPR CNRS 9048, 87 Avenue du Docteur Schweitzer, 33608 PESSAC cedex, France |
| Abstract: | A two-site immunochemiluminometric assay for human growth hormone has been developed based on the use of chemiluminescent acridinium ester labelled monoclonal antibodies and a magnetisable particle - polyclonal antibody solid-phase. The assay has an incubation time of 1 h at room temperature and rapid separation and quantitation stages. The sensitivity of detection is 0.12 mU/l and the working range is 0.88 to greater than 100 mU/l for inter-assay CVs less than or equal to 15%. The assay is convenient both technically and clinically since the wide range of growth hormone levels seen in growth hormone deficiency and acromegaly can be quantified accurately in a single test without the need for repeated sample dilution. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |