华支睾吸虫溶血磷脂酶在虫体组织定位及其特异性抗体类型分析

目的观察华支睾吸虫溶血磷脂酶(CsLysoPLA)在成虫的组织定位,分析其诱导人体产生的特异性抗体类型。方法应用免疫荧光方法,观察CsLysoPLA在成虫的组织定位;应用ELISA方法,CsLysoPLA重组蛋白检测25份华支睾吸虫病人血清的IgG1、IgG2、IgG4、IgE。结果CsLysoPLA定位于虫体的口吸盘、排泄囊、肠支、生殖孔,与虫体分泌/排泄抗原物质密切相关的部位;以CsLysoPLA重组蛋白检测华支睾吸虫感染者血清,IgG1抗体水平较高,IgG2抗体水平稍高,而感染者的IgG4、IgE水平则与健康人无明显差异。结论CsLysoPLA主要经虫体消化道、生殖道、排泄系统分泌/排泄到体外,刺激人体免疫系统产生Th2为主的免疫应答。

The histo-localization of lysophospholipase from Clonorchis sinensis and the isotype analysis for the specific antibody responses

To observe the histo-localization of lysophospholipase from Clonorchis sinensis (CsLysoPLA) and to analyze the isotype of specific antibody responses in humans, the localization of CsLysoPLA from adult worms was demonstrated by immuno-fluorescent techniques ,and the recombinant CsLysoPLA protein was used as antigen in ELISA assay to detect the presence of the specific antibodies of IgG1, IgG2, IgG4 and IgE in serum samples of 25 patients with C. sinensis infection. It was found that CsLysoPLA was localized in the oral sucker, the excretory bladder, the intestine and the genital pore of adult worms. By using the recombinant CsLysoPLA as antigen in ELISA, it was shown that the level of specific antibody IgG1 was rather high and that of IgG2 demonstrated moderate elevation. However, there was no significant difference in the IgG4 and IgE antibody levels between patients and the healthy individuals. From these observations, it is evident that the CsLysoPLA protein is mainly excreted out through digestive and reproductive tracts as well as the excretory system of adult worms and the immune responses induced in humans are essentially of Th2-type responses.