昆明山海棠对胶原性关节炎大鼠免疫功能的干预作用及其机制

目的: 观察昆明山海棠(THH)对胶原性关节炎(CIA)大鼠免疫功能的干预作用,探讨其可能的作用机制。 方法: SD大鼠50只,随机分为正常组(n=10)和CIA模型制备组(n=40),用鸡Ⅱ型胶原(CⅡ)诱导大鼠制备CIA模型。再将造模成功的36只大鼠随机分为模型组、地塞米松组及200和400 mg·kg^-1THH干预组。在实验过程中观察各组大鼠足爪肿胀度和关节炎指数(AI)等变化。实验第35天,采用ELISA法检测血清中抗CⅡ抗体水平;MTT法检测脾T细胞和B细胞增殖活性;ELSIA法检测脾组织白细胞介素12(IL-12)、IL-23和肿瘤坏死因子α(TNF-α)水平;取各组大鼠足爪组织行HE染色,观察组织病理学变化。 结果: 与正常组比较,模型组大鼠AI明显上升(P<0.01),血清抗CⅡ抗体水平、脾T细胞和B细胞增殖活性、脾组织中IL-12、IL-23和TNF-α水平明显升高(P<0.01);大鼠足爪皮下组织细胞排列紊乱且有大量炎性细胞浸润及血管增生。与模型组比较,400 mg·kg^-1THH干预组大鼠一般状况得到明显改善,足爪肿胀度和AI明显下降(P<0.01),脾T细胞和B细胞增殖活性明显下降(P<0.01),血清中抗CⅡ抗体水平明显降低(P<0.01),脾组织中IL-23、TNF-α和IL-12水平均明显降低(P<0.01),大鼠足爪组织病理学改变明显减轻。200 mg·kg^-1THH组大鼠上述指标无明显变化(P>0.05)。 结论: 高剂量(400 mg·kg^-1)THH对CIA大鼠关节炎有明显抑制作用,其机制可能是THH能明显抑制T和B淋巴细胞的增殖反应,并抑制脾组织中IL-23、TNF-α和IL-12和血清中抗CⅡ抗体水平,进而明显改善大鼠足爪组织病理学变化。

Intervention effect of tripterygium hypoglaucum hutch on immune function of rats with collagen induced arthritis and its mechanism

Objective: To investigate the intervention effect of tripterygium hypoglaucum hutch(THH)on the immune function of rats with collagen induced arthritis(CIA),and to clarify its possible mechanism.Methods Fifty SD rats were randomly divided into normal group(n=10)and CIA group(n=40).The rats in CIA group were induced into CIA models by type Ⅱ collagen. 36 rats were successfully set up and randomly divided into model group,dexamethasone group,200 mg·kg^-1 THH group and 400 mg·kg^-1 THH group(n=9).The degree of swelling of paw and the articular index changes of the rats in various groups during the experiment were observed.On the 35th day of experiment,the level of anti-C Ⅱ IgG antibody was detected by ELISA;the proliferation activities of spleen T and B lymphocytes were detected by MTT;the levels of interleukin-12(IL-12),interleukin-23(IL-23)and tumor necrosis factor-α(TNF-α)in spleen tissue were determined by ELISA.The paw tissue was collected and stained by HE,and the histopathological changes were observed.Results: Compared with normal group,the articular index of the rats in model group was significantly increased(P<0.01);the levels of serum anti-C Ⅱ IgG antibody,the proliferation activities of spleen T and B lymphocytes,the levels of IL-12,IL-23 and TNF-α in spleen tissue of the rats in model group were significantly increased(P<0.01);the subcutaneous tissue cells of the rat paw in model group arranged irregularly with inflammatory cells infiltrating and vascular proliferation.Compared with model group,the general situation of the rats in 400 mg·kg^-1 THH group was improved;the degree of swelling of paw and the articular index of the rats were reduced(P<0.01),and the proliferation activities of spleen T and B lymphocytes were significantly decreased(P<0.01);the levels of serum anti-CⅡ IgG antibody was significantly decreased(P<0.01);the levels of IL-23,TNF-α and IL-12 in spleen tissue were significantly decreased(P<0.01);the histopathological changes of rat paw were significantly improved.There were no significant changes in the above indicators in 200 mg·kg^-1 THH group. Conclusion: 400 mg·kg^-1 THH has significantly inhibitory effect on arthritis in the CIA rats,and its possible mechanisms may be related to obviously inhibiting the proliferation of T and B lymphocytes and significantly inhibiting the levels of IL-23,TNF- a,IL-12 in spleen tissue and serum anti-C Ⅱ antibody and significantly improving the pathological changes of the paw tissue of rats.