FADS1 rs174537基因多态性与侵袭性牙周炎患者血清蛋白的相关性

目的：探讨FADS1 rs174537基因多态性与侵袭性牙周炎患者血清蛋白的相关性，为侵袭性牙周炎的诊断和治疗提供一定的理论基础。方法：选择353名侵袭性牙周炎患者（AgP组）和125名健康对照者（HP组）进行FADS1 rs174537位点基因型和血清生化指标的检测，比较分析不同FADS1 rs174537基因型患者的血清总蛋白（total protein ,TP）、球蛋白（globulin，GLB）、白蛋白（albumin，ALB）、白球比（albumin/globulin，A/G）。结果： （1）AgP组FADS1 rs174537位点G等位基因检出率显著高于HP组（68.1% vs. 61.2%, P=0.046，OR=1.35，95%可信区间为1.00~1.83）；AgP组FADS1 rs174537位点GG基因型的检出率显著高于HP组（45.5% vs.34.4%， P=0.029，OR=1.60，95%可信区间为1.05~2.44）；（2）AgP组GG基因型患者的TP、GLB水平显著低于GT+TT基因型患者［TP (77.08±7.88) g/L vs. (79.00±4.66) g/L，P=0.007；GLB (28.17±7.63) g/L vs. (29.88±3.49) g/L，P=0.007］，A/G则显著高于GT+TT基因型患者（1.72±0.22 vs.1.67±0.22，P=0.040），而ALB水平差异没有统计学意义（P>0.05）， HP组不同基因型者的TP、GLB、A/G及ALB差异均无统计学意义（P>0.05）；（3）AgP组GT+TT基因型患者的TP、GLB较相同基因型的健康受试者显著升高 ［TP (79.00±4.66) g/L vs. (75.20 ±4.53) g/L, P<0.01；GLB (29.88±3.49) g/L vs.(26.55±2.94) g/L, P<0.01］， A/G更低水平（1.67±0.22 vs. 1.88±0.30, P<0.01），差异具有统计学意义, 而ALB水平差异无统计学意义（P>0.05）；AgP组GG基因型患者与HP组GG基因型者相比，TP、GLB、A/G及ALB差异均无统计学意义（P>0.05）。结论： FADS1 rs174537位点与侵袭性牙周炎的易感性密切相关，在侵袭性牙周炎患者中，该位点GG基因型与低TP、GLB、高A/G有关；FADS1 rs174537位点的GG基因型可能通过降低牙周防御机能，促进炎症反应，从而成为侵袭性牙周炎的重要危险因素之一。

Association between FADS1 rs174537 polymorphism and serum proteins in patients with aggressive periodontitis

Objective:To investigate the potential association between FADS1 rs1 74537 polymorphism and serum proteins in patients with aggressive periodontitis,which may provide benefits for diagnosis and treatment of aggressive periodontitis.Methods:A total of 353 patients with aggressive periodontitis (group AgP)and 1 25 matched controls (group HP)were recruited in the study.Genotyping of FADS1 rs1 74537 and serum biochemical indexes were tested at the study’s start.The relationships between the levels of TP,GLB,ALB,A/G and genotyping were analyzed.Results:(1 )The detection rate of allele G in group AgP was higher than that in group HP(68.1% vs.61 .2%,P=0.046,OR=1 .35,95% CI 1 .00-1 .83 );the detection rate of genotype GG in group AgP was higher than in group HP(45 .5%vs. 34.4%,P=0.029,OR=1 .60,95%CI 1 .05 -2.44).(2)In group AgP,the patients with GG geno-type exhibited significantly lower TP,GLB than the patients with GT+TT genotype (77.08 ±7.88)g/L vs.(79.00 ±4.66)g/L,P=0.007;(28.1 7 ±7.63)g/L vs.(29.88 ±3.49)g/L,P=0.007)and the higher A/G(1 .72 ±0.22 vs.1 .67 ±0.22,P=0.040),but there was no significant difference in ALB between the patients with GG genotype and the patients with GT+TT genotype.In group HP,there were no significant differences in TP,GLB,A/G and ALB between individuals with genotype GT+TT and with genotype GG.(3 )Compared with individuals with genotype GT+TT in group HP,the AgP pa-tients with genotype GT +TT exhibited significantly higher TP,GLB (79.00 ±4.66 ) g/L vs. (75.20 ±4.53)g/L,P<0.01;(29.88 ±3.49)g/L vs.(26.55 ±2.94)g/L,P<0.01 )and the lo-wer A/G(1 .67 ±0.22 vs.1 .88 ±0.30,P<0.01 ),but there was no significant difference in ALB. There were no significant differences in TP,GLB,A/G and ALB the between the AgP patients with ge-notype GG and the healthy subjects with the same genotype either.Conclusion:FADS1 rs1 74537 poly-morphism is associated with aggressive periodontitis.The patients with genotype GG in group AgP had relatively lower TP,GLB and higher A/G.Genotype GG might be a risk indicator for aggressive periodon-titis by reducing host defense capability and contributing to inflammatory response in the occurrence and development of aggressive periodontitis.