NRP1和miR-9在人NSCLC组织和癌旁组织中的表达及其临床意义

目的: 检测人非小细胞肺癌(NSCLC)组织标本中神经纤毛蛋白1(NRP1)mRNA和miR-9的表达水平,并探讨二者表达与NSCLC患者临床特征之间的关系。方法: 在患者知情同意下,收集2010-2011年吉林大学中日联谊医院匹配的NSCLC患者组织标本,包括45例正常组织、45例癌旁组织及45例肿瘤组织。采用qRT-PCR法检测不同组织中NRP1 mRNA和miR-9表达水平,分析二者表达水平与不同临床病理特征的相关性。结果: 与正常组织比较,癌旁组织中NRP1 mRNA表达水平无明显变化(P>0.05),而肿瘤组织中NRP1 mRNA表达水平明显降低(P<0.05);与正常组织比较,癌旁组织miR-9表达水平无明显变化(P>0.05),而肿瘤组织中miR-9表达水平明显升高(P<0.05)。男性癌旁组织中miR-9表达水平低于女性(P<0.05)。NSCLC患者肿瘤组织中NRP1 mRNA表达水平与性别、年龄、分化程度、TNM分期及临床分期无关联(P>0.05),但与病理分型及淋巴结转移有关联(P<0.05);NSCLC患者肿瘤组织中miR-9表达水平与年龄、肿瘤病理类型、淋巴转移、分化程度、TNM分期及临床分期无关联(P>0.05),而与性别因素有关联(P<0.05)。结论: NSCLC患者肿瘤组织中miR-9表达水平显著升高,而NRP1 mRNA表达水平明显下降,NRP1 mRNA检测有助于判断NSCLC的分型及转移。

Expressions of miR-9 and NRP1 in human NSCLC tissue and adj acent carcinoma tissue and their clinical significances

Objective:To detect the expression levels of neuropilin1 (NRP1)mRNA and miR-9 in non-small cell lung cancer (NSCLC)tissue samples, and to explore the correlations between the expressions of NRP1 mRNA, miR-9 and the clinicopathological characteristics of the patients with NSCLC.Methods:Informed consent was obtained from each patient before surgery.The tissue samples including 45 NSCLC tissue ,45 adjacent carcinoma tissue and 45 normal lung tissue were collected from China-Japan Union Hospital of Jilin University from 2010 to 2011.qRT-PCR was used to detect the expression levels of NRP1 mRNA and miR-9 in three kinds of lung tissue, and the correlation between the expressions of NRP1 mRNA, miR-9 and clinicopathological characteristics of the patients with NSCLC was analyzed.Results:Compared with normal tissue,the expression level of NRP1 mRNA in adjacent carcinoma tissue had no change (P>0.05),but the expression level of NRP1 mRNA in non-small cell lung cancer tissue was significantly decreased (P<0.05).Compared with normal tissue,the expression level of miR-9 in adjacent carcinoma tissue had no change (P>0.05),but the expression level of miR-9 in non-small cell lung cancer tissue was significantly increased (P < 0.05 ). Furthermore, in adjacent carcinoma tissue, the expression level of miR-9 in the males was lower than that in the females (P<0.05 ). In NSCLC tissue, the expression level of NRP1 mRNA had no relationship with sex,age,differentiation degree,TNM stage and clinical stage,but was significantly correlated to the histological subtype and lymph node metastasis (P<0.05).In NSCLC tissue,the expression level of miR-9 had no relationship with age, pathological type, lymph node metastasis, differentiation degree,TNM stage,and clinical stage (P>0.05),but was correlated to the sex (P<0.05). Conclusion:The expression level of miR-9 is up-regulated and the expression level of NRP1 mRNA is down-regulated significantly in non-small cell lung cancer tissue. The detection of the expression level of NRP1 mRNA contributes to j udge the histological subtype and lymph node metastasis of NSCLC.