神经干细胞移植对大鼠脑自由落体撞击伤的治疗作用

目的通过神经干细胞(NSCs)移植治疗大鼠脑自由落体撞击伤,探讨NSCs对脑外伤功能恢复的影响。方法体外培养NSCs并用5-2溴脱氧尿嘧啶(B rdU)标记。自由落体撞击大鼠左侧大脑皮质运动感觉区以制作脑外伤模型,脑外伤后24h内移植NSCs,分别于脑外伤前、脑外伤后1、2、4周时行神经运动行为学评分(NMFE)和免疫组织化学染色检测B rdU、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)和半乳糖脑苷酯(GalC)蛋白表达。结果大鼠自由落体撞击伤后,右下肢神经运动功能障碍明显。神经运动行为学评分在第1周时,实验组与对照组无明显差异,而在第2、4周时,实验组的评分明显低于对照组,差异有统计学意义(P<0.05)。第2、4周时实验组NSE、GFAP和GalC染色阳性细胞数多于对照组,nestin染色在第1周时表达最高,以后逐渐下降。B rdU阳性细胞在损伤灶中心区最高多,在损伤灶的远隔部位也有少许发现。结论NSCs移植有利于大鼠脑自由落体撞击伤后期的功能恢复。移植的NSCs可以在损伤部位存活并增殖分化与迁移。

Effects of neural stem cells on rat brain weight-dropping injury

Objective To investigate the effects of embryonic neural stem cells (NSCs)on the repair of rat brain weight-dropping injury.Methods NSCs were cultured in vitro,labeled with BrdU,and transplanted into rat brain area injured by weight-dropping.The neurological motor function evaluation(NMFE) were used to test the functions at the 1,2,4 weeks post-injury.Immunohistochemistry was used to detect the expressions of BrdU,nestin,NSE,GFAP and GalC.Results There was significant difference in NMFE between experiment group and control group(P <0.05)at 2,4 weeks.At 2,4 week,there were more expressions of NSE,GFAP and GalC in experiment group than in control group.Expression of nestin was higher at 1 week than at 2,4 week.BrdU positive cells were mainly detectable in traumatic area and simultaneously less at the distance from traumatic area using immunohistochemical staining.Conclusion NSCs have effects on rat brain weight-dropping injury.Transplanted NSCs can survive,proliferate, differentiate and immigrate in traumatic area.