| 人胆囊癌CD133+细胞亚群致瘤性的实验研究 |
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| 引用本文： | 石程剑,秦仁义,石秀春,田锐,王敏,宫伟强.人胆囊癌CD133+细胞亚群致瘤性的实验研究[J].肿瘤,2010,30(9). |
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| 作者姓名： | 石程剑 秦仁义 石秀春 田锐 王敏 宫伟强 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院胆胰外科,武汉,430030
2. 湖南省湘西自治州人民医院肝胆外科,吉首,416000 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:研究CD133在人胆囊癌细胞中的表达,初步探讨胆囊癌CD133+亚群的致瘤能力.方法:将人原代胆囊癌组织植入裸鼠皮下形成移植瘤,并同人原代胆囊癌组织分别制成单细胞悬液;FCM法检测CD133的表达情况,并分选出CD133+和CD133-亚群;对不同亚群细胞进行体外克隆形成实验和裸鼠体内移植瘤形成实验,免疫组织化学法验证移植瘤的组织表型.结果:FCM法检测显示,胆囊癌细胞中1.95%～3.24%的细胞CD133呈阳性表达;体外培养显示CD133+亚群比CD133-亚群具有更强的克隆球形成能力,在裸鼠体内也具有更强的肿瘤形成能力(P<0.05);免疫组织化学检测结果提示,胆囊癌CD133+细胞形成的移植瘤同人原代胆囊癌具有相同的组织表型,均表达CA19-9和CD44s.结论:人胆囊癌CD133+细胞亚群具有高致瘤性,其中可能富含有肿瘤干细胞.
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| 关 键 词： | 胆囊肿瘤 肿瘤干细胞 肿瘤移植 抗原 CD133 致瘤性 |
Tumorigenicity of the CD133+ population in human gallbladder carcinomas |
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| SHI Cheng-jian,QIN Ren-yi,SHI Xiu-chun,TIAN Rui,WANG Ming,GONG Wei-qiang.Tumorigenicity of the CD133+ population in human gallbladder carcinomas[J].Tumor,2010,30(9). |
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| Authors: | SHI Cheng-jian QIN Ren-yi SHI Xiu-chun TIAN Rui WANG Ming GONG Wei-qiang |
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| Institution: | SHI Cheng-jian1,QIN Ren-yi1,SHI Xiu-chun2,TIAN Rui1,WANG Ming1,GONG Wei-qiang1(1.Department of Biliary-pancreatic Surgery,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China,2.Department of Hepatobiliary Surgery,Xiangxi Autonomous Prefecture People's Hospital of Hunan Province,Jishou 416000,China) |
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| Abstract: | Objective:To study CD133 expression in human gallbladder carcinomas and the tumorigenicity of the CD133+ subpopulation.Methods:The human gallbladder cancer tissues were subcutaneously transplanted into nude mice to generate xenograft tumors.Gallbladder cancer cells were isolated to prepare single cell suspension.The expression of CD133+ population was detected using flow cytometry.The CD133+ and CD133-subpopulations were selected and isolated.The in vitro clone formation test and in vivo xenograft formation... |
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| Keywords: | Gallbladder neoplasms Tumor stem cells Neoplasms transplantation Antigens CD133 Tumorigenicity |
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