Analysis of interleukin-8 secretion induced by Helicobacter pylori from the gastric epithelial cell line MKN45: A mechanism independent of the intensity of cytotoxicity |
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| Authors: | ATSUSHI TAKAGI SHIGERU KAMIYA YASUHIRO KOGA UKI OHTA HIROHISA KOBAYASHI TAKAYUKI SHIRAI SHIGERU HARASAWA TAKESHI MIWA |
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| Affiliation: | Department of Internal Medicine 6, Tokai University School of Medicine, Kanagawa;†Infectious Diseases, Tokai University School of Medicine, Kanagawa;*Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan |
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| Abstract: | Interleukin (IL)-8, a potent chemoattractant and activator of neutrophils, has been implicated to have a major role in the pathogenesis of gastric mucosal injury by Helicobacter pylori infection. We examined the relationship between cytotoxicity and IL-8 secretion induced by H. pylori. Furthermore, whether the vacuolating cytotoxin of H. pylori mediates IL-8 secretion from gastric epithelial cell lines was examined. Among the inflammatory cytokines, messages for IL-6, IL-8 and transforming growth factor-β1 were produced by gastric cancer (MKN45) cells in response to exposure to the cytotoxic strain of H. pylori. MKN45 incubated with the viable cytotoxic strain of H. pylori. secreted IL-8. In contrast, the supernatant of neither the cytotoxic nor the non-cytotoxic strain induced IL-8 secretion. There was no correlation between IL-8 secretion and the intensity of cytotoxicity. In conclusion, these findings suggest that IL-8 secretion from MKN45 induced by H. pylori is mediated by factors other than cytotoxicity. |
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| Keywords: | gastric cancer cell line, Helicobacter pylori, interleukin-8, vacuolating cytotoxin. |
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