四君子汤对脾虚证大鼠胃黏膜黏液凝胶表达的影响

目的]探讨脾虚证大鼠胃黏膜黏液凝胶（MUC5AC）的变化情况，以及健脾益气中药四君子汤对其改变的影响。方法]除空白对照组大鼠外，其余3组均采用复合因素造模法复制脾虚证动物模型。脾虚证模型组造模7周；自然复健组、四君子汤组造模4周后常规喂养3周，四君子汤组同时用四君子汤进行治疗。用Western bolt方法检测观察各组大鼠胃组织中MUC5AC表达的变化。rN果]与空白对照组比较，脾虚证模型组、自然复健组大鼠胃组织中MUCSAC含量明显减少（P〈0.05），四君子汤组的MUC5AC含量明显增加（P〈0．05）。结论]脾虚证可以通过对胃黏膜的防御机制的微观指标产生影响，从而导致胃黏膜屏障功能的下降，进一步导致慢性胃病的发生；四君子汤可通过改善胃黏膜屏障功能，从而对慢性胃病起到治疗作用。

Sijunzi decoction on gastric MUC5AC expression of spleen deficiency rats

Objective]To investigate the gastric MUCSAC expression in spleen deficiency rats, and to detect the effect of Sijunzi decoction （SD） on the expression, l-Methods]Composite factors were used to du- plicate the animal model of spleen deficiency and SD was applied for the treatment. MUC5AC expression was detected by western bloting in rat stomach tissue. Results] MUCSAC expression was decreased signif- icantly in spleen deficient models （P〈0. 05） ,and after given SD treatment,MUCSAC expression increased significantly （P〈0. 05）. Conclusion]Spleen deficiency has micro-changes on the gastric mucosa which lead to its decreased barrier function. SD, a kind of herbal composition with effect of strengthening spleen Qi, can improve the micro-changes such as MUCSAC expression and treat the chronic gastric diseases.