IL—1对输精管结扎大鼠睾丸间质细胞功能影响的体外研究

本文观察ＩＬ－１对正常和输精管结扎大鼠睾丸间质细胞（Ｌｅｙｄｉｇｃｅｌｌｓ，莱迪希细胞）细胞功能的影响，结果表明：在人绒毛膜促性腺激素（ｈｕｎａｎｃｈｏｒｉｏｍｉｃｇｏｎａｄｏｔｒｏｐｉｎｈＣＧ）的作用下，正常和输精管结扎大鼠睾丸Ｌｅｙｄｉｇ细胞培养上清中睾酮和ｃＡＭＰ的含量均明显高于相应的对照孔，且两组比较无明显差异，说明输精管结扎后睾丸Ｌｅｙｄｉｇ细胞对垂体激素的反应性并无改变，两组细胞受ＩＬ

Effect of IL- 1 on function of testis Leydig cells of vasectomized rat in vitro

Effect of IL-1 on function of testis Leydig cells in normal and vasectomized rat in vitro was observed on the basis of our previous experiments. The results showed that contents of testosterone and cAMP in supernate in testis leykig cells in normal and vasectomized rats increased obviously as hCG was used as a stimulating factor, there was no significant difference between 2 groups, which indicated that there was no change in response of testis Leydig cells on pituitary hormone after vasectomy; the result was the same as above while IL -1 was stimulating factor. Testosterone content in supemate decreased obviously as hCG and IL- 1 were added into culture in 2 groups.This illustrated that IL-1 regulated and inhibited biological synthsis of testos terone induced by hCG in Leydig cells.Therefore,it would be very important to dectease IL-1 locally in testis to prevent the decrease of testosterone.