NDP-MSH inhibits neutrophil migration through nicotinic and adrenergic receptors in experimental peritonitis |
| |
Authors: | Jozi Figueiredo Ana Elisa Ferreira Rangel Leal Silva Luis Ulloa Paolo Grieco Thiago Mattar Cunha Sérgio Henrique Ferreira Fernando de Queiróz Cunha Alexandre Kanashiro |
| |
Institution: | 1. Department of Pharmacology, Faculty of Medicine of Ribeir?o Preto, University of S?o Paulo, Av. Bandeirantes 3900, 14049-900, Ribeir?o Preto, S?o Paulo, Brazil 2. Laboratory of Anti-Inflammatory Signaling and Surgical Immunology, Department of Surgery, UMDNJ–New Jersey Medical School, 185 South Orange Avenue, 07103, Newark, NJ, USA 3. Department of Pharmaceutical and Toxicological Chemistry, University of Naples “Federico II”, 80131, Naples, Italy
|
| |
Abstract: | Melanocortin is a potent anti-inflammatory molecule. However, little is known about the effect of melanocortin on acute inflammatory processes such as neutrophil migration. In the present study, we investigated the ability of Nle4, D-Phe7]-melanocyte-stimulating hormone (NDP-MSH), a semisynthetic melanocortin compound, in the inhibition of neutrophil migration in carrageenin-induced peritonitis model. Herein, subcutaneous pretreatment with NDP-MSH decreased neutrophil trafficking in the peritoneal cavity in a dose-dependent manner. NDP-MSH inhibited vascular leakage, leukocyte rolling, and adhesion and reduced peritoneal macrophage inflammatory protein 2, but not TNF-alpha, IL-1beta, IL-10, and keratinocyte-derived chemokine production. In addition, the effect on neutrophil migration was reverted by the pretreatment with both propranolol (a nonselective beta-adrenergic antagonist) and mecamylamine (a nonselective nicotinic antagonist) but not by splenectomy surgery. Moreover, NDP-MSH intracerebroventricular administration inhibited neutrophil migration, indicating participation of the central nervous system. Our results propose that the NDP-MSH effect may be due to a spleen-independent neuro-immune pathway that efficiently regulates excessive neutrophil recruitment to tissues. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|