发现2个FGFR1细胞外段同源的cDNA片段

目的：克隆表达人成纤维细胞生长因子受体。方法：采用ＲＴ－ＰＣＲ法，结果：在钓取人ｃＤＮＡ的过程中，意外地钓取到２个与ＦＧＦＲ１细胞外段同源的ｃＤＮＡ片段，经克隆和ＤＮＡ序列分析发现它们是ＦＧＦＲ１细胞外源的ｃＤＮＡ片段，。结论：虽然对这个２个ｃＤＮＡ片段的功能尚不明确，但这种抓住新现象并进行探究的意识对科研很重要。

Discovery of two FGFR1 extracellular domain homologous-cDNA fragments

Objective:To clone and express fibroblast growth factor receptor1 Methods:RT PCR is used to isolate FGFR1 cDNA Results:In the process of isolating the extracellular domain cDNA of fibroblast growth factor receptor 1 (FGFR1) from human lung fibroblasts, discovered two cDNA species After analysis of the cDNAs by cloning and sequencing,have indentified that the two species are FGFR1 extracellular domain homologous cDNA Conclusion:Don't know the function of the two cDNA but it is very important that a net phenomenon in the research should be taken and studied Here only present the discovery of the isoforms whose functions will be studied in the future