| 檀香萜烯合成酶基因的克隆与序列分析 |
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| 引用本文： | 文海涛,赵红英,林励,邱贤秀. 檀香萜烯合成酶基因的克隆与序列分析[J]. 广东药学院学报, 2010, 26(2): 131-133. DOI: 10.3969/j.issn.1006-8783.2010.02.006 |
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| 作者姓名： | 文海涛 赵红英 林励 邱贤秀 |
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| 作者单位： | 广州中医药大学,中药学院,广东,广州,510006;广州中医药大学,中药学院,广东,广州,510006;广州中医药大学,中药学院,广东,广州,510006;广州中医药大学,中药学院,广东,广州,510006 |
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| 基金项目： | 高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的通过克隆技术获得参与檀香挥发油形成的萜烯合成酶基因。方法利用CTAB-LiCl法提取檀香已结香心材总RNA,采用RT-PCR技术克隆萜烯合成酶基因。结果克隆获得了一个檀香萜烯合成酶基因,该基因编码区全长1 731 bp,编码576个氨基酸残基。结论 CTAB-LiCl法能提取较高质量的檀香心材总RNA,克隆获得的萜烯合成酶具有编码区。
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| 关 键 词： | 檀香 萜烯合成酶 基因克隆 序列分析 |
Cloning and sequence analyzing of terpene synthase gene in Santalum album L. |
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| WEN Hai-tao,ZHAO Hong-ying,LIN Li,QIU Xian-xiu. Cloning and sequence analyzing of terpene synthase gene in Santalum album L.[J]. Academic Journal of Guangdong College of Pharmacy, 2010, 26(2): 131-133. DOI: 10.3969/j.issn.1006-8783.2010.02.006 |
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| Authors: | WEN Hai-tao ZHAO Hong-ying LIN Li QIU Xian-xiu |
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| Affiliation: | (School of Chinese Materia Medica,Guangzhou University of TCM,Guangzhou,Guangdong 510006,China) |
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| Abstract: | Objective To clone the terpene synthase gene from Santalum album L.which participates in the synthesis of essential oil.Methods CTAB-LiCl treatment was used to extract total RNA from the heartwood of Santalum album L.And RT-PCR was employed to clone the terpene synthase gene at the same time.Results One terpene synthase gene consisted of 1 731 bp nucleic acid was cloned from Santalum album L.,which encodes 576 amino acid residues.Conclusion CTAB-LiCl treatment could extract high quality RNA from the heartwood of Santalum album L.,and the cloned terpene synthase gene had the open reading frame. |
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| Keywords: | Santalum album L. terpene synthase gene cloning sequence analysis |
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