RP-HPLC中系统因素对奥赛博司色谱行为的影响

考察不同流动相pH、色谱柱和柱温对奥赛博司RP—HPLC色谱行为的影响。方法：色谱柱：Waters SunFire和Kromasil 100—5RPC18（500mm×4．6mm，5μm）；流速：1ml·min^-1；检测波长：220nm；进样量：20μ1；柱温：（25，28，30，35℃）；流动相：磷酸盐缓冲溶液（0．1mol·L^-1。KH2PO4＋0．02mol·L^-1H3PO4）乙腈=8：2（磷酸盐缓冲溶液用磷酸二氢钾饱和溶液调pH分别为：2．83，2．84，2．95，2．99，3．02，3．05，3．07，3．14，4．0，4．50，5．0，5．50，6．05）。结果：两种色谱柱、不同柱温对奥赛博司的色谱行为没有太大影响；但当流动相pH不同时对其有一定影响。结论：本方法可为奥赛博司质量标准的系统研究提供依据。

Effect of RP-HPLC Systematic Factors on the Chromatographic Behavior of OSAIBOS

To study the effect of various pH value of mobile phase, different columns and column temperature on the chromatographic behavior of OSAIBOS. Method： The chromatographic separation was performed on RPC18 （500 mm×4.6ram ,5μm） of Waters SunFire and Kromasil 100-5 with the column temperature at 25, 28, 30, 35℃ respectively and detection wavelength at 220nm, the flow rate was set at 1 ml·min^-1 and 20μ1 of sample was injected for analysis; the mobile phase consisted of a phosphate buffer solution （0.1 mol·L^-1 potassium dihydrogen phosphate ＋0.02 mol·L^-1 Phosphoric acid adjusted pH with potassium dihydrogen phosphate saturated solution to 2.83,2.84,2.95,2.99,3.02,3.05,3.07,3.14,4.0,4.50,5.0,5.50,6.05 respectively） ： acetonitrile = 8： 2. Result： These two chromatographic columns and different column temperature have no significant influence on the chromatographic behavior of OSAIBOS, but the pH value of mobile phase does. Conclusion ： This method can provide bases for the systematic study of quality criteria of OSAIBOS.