尾静脉注射骨髓细胞对糖尿病小鼠视网膜新生血管化的治疗作用

目的:研究尾静脉注射骨髓细胞对于链脲佐菌素(strepto-zotocin,STZ)诱导的糖尿病(diabetes mellitus,DM)小鼠视网膜振荡电位(oscillatory potentials,OPs)和视网膜新生血管密度的影响。方法:40只C57BL/6J小鼠随机分成正常对照(NC)组和DM造模组。DM造模组给予多性腹腔注射,造模成功后再随机分为DM对照组和DM骨髓治疗组,饲养16wk,罗兰视觉电生理仪检测其OPs波,视网膜铺片联合免疫荧光染色检测视网膜新生血管密度。DM骨髓治疗组给予小鼠骨髓细胞悬液尾静脉注射,注射2wk和8wk后再次分别检测OPs波及视网膜新生血管密度。结果:高血糖的影响使得DM小鼠造模成功后OPs波振幅呈明显降低趋势,OPs峰潜时明显延长,与对照组小鼠各时期相比有显著差异(P<0.05)。骨髓治疗2wk和8wk后即小鼠26wk及32wk时,DM骨髓治疗组较DM组OPs波幅明显升高,OPs峰潜时明显缩短,有显著差异(P<0.05)。而26wk及32wk时NC组及DM骨髓治疗两组OPs振幅及峰潜时相比无显著差异(P>0.05)。视网膜铺片正常对照组血管发育成熟,血管网结构清晰,DM对照组血管迂曲阻塞,大量结构异常新生血管,丧失了血管网结构,DM骨髓治疗组见血管网结构清晰,仍有少部分深层血管阻塞,形态接近正常小鼠。结论:骨髓细胞移植可以有效抑制视网膜新生血管形成。

Therapeutic effect of retinal neovascularization in diabetic mice by injection of isolated bone marrow cell through vena caudalis

AIM:To investigate the change of the oscillatory potentials(OPs) and dense of retinal neovascularization(RNV) of diabetes mellitus(DM) rats after injection of isolated bone marrow cell through vena caudalis. ·METHODS:Forty C57BL/6J rats were divided at random to receive streptozotocin(50mg/kg) intraperitoneally(diabetes) or cit rate buffer alone (normal control). One week after injections,only streptozotocin-treated rats with plasma glucose concentrations above 16.7mmol/L were considered to be diabetic and were subsequently divided into two DM group and bone marrow(BM)-treated group. At the end of the 16th week,the OPs of the rats in three groups were detected by Roland Electrophysiological Instrument and the dense of RNV measured by immunohistochemical examination.Afterward the BM-treated group received injection of BM suspension of rats via vena caudalis and the OPs and dense of RNV were reexamined at the end of 18th and 24th week,respectively. ·RESULTS:After 16 weeks from models,the amplitude of ΣOP(sum of OP1,OP2,OP3 and OP4) and OP TTP (sum of P1,P2,P3 and P4) were significantly lower than those in normal control group(P<0.05),which recovered after injection of isolated bone marrow(P<0.05).There was no statistical difference between BM-treated group and control group(P>0.05).Immunohistochemical examination showed that the vasculature was distinctly displyed in normal control group; a lot of curved blocked vessels and retinal neovascularization make the retina lose its original structure in DM group;while vasculature was clearly demonstrated but there was still blockage of vessels in BM-treated group. ·CONCLUSION:Vena caudalis injection of isolated bone marrow cell can inhibit retinal neovascularization.