棉酚对大白鼠精子发生影响的银染观察

大白鼠喂服棉酚后,对其附睾精子活动力、精子形态学及睾丸减数分裂制片进行银染观察。对大白鼠一次性喂服大剂量棉酚(400 mg/kg体重),喂后2～5周,精子活动力明显降低,不正常精子频率增加,附睾精子银染显示精子尾部有损伤,包括精子头尾分离、尾部成圈、精子尾部中段部分区域损伤,但精子头部形态正常。服药6周后,附睾精子全部恢复正常。短期体外孵育实验,显似类似结果,但较轻微。喂服棉酚2～4周期间,不同发育阶段精子细胞的嗜银结构消失。实验组睾丸组织切片及睾丸减数分裂制备片,并无异常发现。

CYTOLOGICAL EFFECT OF GOSSYPOL ON RAT GAMETOGENESIS AND SPERMATOZOA

Induction of possible male sterility in laboratory rats by gossypol was examined by microscopic observations on sperm motility, sperm morphology following silver staining, and meiosis. The rats were force-fed with gossypol acetate once with 400mg/kg body weight. In a span of 2-5 weeks post-feeding, a marked reduction of sperm motility was observed with a concurrent increase of abnormal spermatozoa Silver staining of the spermatozoa showed various tail abnormalities, including disrupted connecting piece, bent tails, looped tails, and cracked midpiece, Sperm head structure appeared to be not affected. Beginning 6 weeks post-feeding, there was a complete recovery. Short-term in vitro experiments showed similar but weaker response. In all samples, histologieal sections and cytogenetic preparations of testes revealed no abnormalities in the activity and normally of meiotic divisions. In animals 2-4 weeks after feeding with gossypol, no argentophilic accessary structures in developing mammalian spermatids were observed.