| 沉默survivin基因表达对人宫颈癌hela细胞恶性行为的影响 |
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| 引用本文： | 鲁艳明,孟丽荣,银铎,王宁,张淑兰. 沉默survivin基因表达对人宫颈癌hela细胞恶性行为的影响[J]. 解剖科学进展, 2011, 17(3): 259-263 |
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| 作者姓名： | 鲁艳明 孟丽荣 银铎 王宁 张淑兰 |
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| 作者单位： | 1. 中国医科大学附属盛京医院妇产科,沈阳,110004
2. 澳门理工学院高等卫生学校病理生理教研室,澳门,00053 |
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| 基金项目： | 国家青年科学基金项目基金资助，辽宁省博士科研启动项目基金资助，中国医科大学附属盛京医院科研项目资助 |
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| 摘 要： | 目的 研究小于扰BNA(small interfering RNA,siRNA):抑制survivin基因的表达对人宫颈癌Hela细胞凋亡的影响.方法 根据survivin基因设计序列特异性的siRNA(survivin-siRNA).在脂质体介导下转染Hela细胞,Western blot方法检测survlvin蛋白...
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| 关 键 词： | 人宫颈癌 凋亡 survivin caspase-3 caspase-7 |
Effect of specific inhibition of survivin gene expression by small interfering RNA on the malignant behavior of human cervical cancer cell line Hela |
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| LU Yan-ming,MENG Li-rong,YIN Duo,WANG Ning,ZHANG Shu-lan. Effect of specific inhibition of survivin gene expression by small interfering RNA on the malignant behavior of human cervical cancer cell line Hela[J]. Progress of Anatomical Sciences, 2011, 17(3): 259-263 |
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| Authors: | LU Yan-ming MENG Li-rong YIN Duo WANG Ning ZHANG Shu-lan |
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| Affiliation: | 1 (1.Department of Gynaecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang 110004 China; 2. Department of Pathophysiology, School of Health Sciences, Macao Polytechnic Institute, Macao 000853 China) |
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| Abstract: | Objective To study the effect of specific inhibition of survivin gene expression by small interfering RNA（siRNA） on the apoptosis of human cervical cancer cell line Hela. Methods The survivin siRNA was designed by web based software, synthesized and then transfected into hela cell line by lipofectamine 2000 reagent. The survivin gene expression inhibition and the expression of caspase-3 and caspase-7 proteins was assessed by Western blot, the apoptosis rate and cell cycle were assessed by TUNEL, Annexin-V and flow cytometry. Results Significant downregulation of the survivin protein expression level was found after transfection of the survivin-siRNA in Hela cells. The apoptosis rate of Hela cells was notably increased, Hela ceils were arrested in G2/M phase, the protein levels of caspase-3 and caspase-7 proteins were higher in siRNA transfected hela cells compared with the untransfected cells and negative control transfected cells. Conclusion Transient transfection of synthesized survivin-siRNA in vitro can downregulate the expression of survivin protein in Hela ceils and induce the apoptosis of hela cells, which may be related to the upregulation of caspase-3 and caspase-7 expressions. |
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| Keywords: | survivin caspase-3 caspase-7 |
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