染铅对大鼠端脑皮质4种生化指标的影响

为研究染铅对大鼠端脑皮质一氧化氮 (NO)、一氧化氮合酶 (NOS)、超氧化物歧化酶 (SOD)、丙二醛(MDA)的影响 ,进一步探明铅的神经毒作用机理。选择Wistar大鼠采用饮水加 0、18 4、184mg L醋酸铅法染毒。结果显示 :与对照组相比 ,低、高剂量组染铅 7d时NOS活力明显升高 (P <0 0 5 )。低剂量组 90d、高剂量组 30d后NOS活力均显著下降 (P <0 0 5 )。 90d时低剂量组NO含量显著低于对照组 (P <0 0 5 ) ,高剂量组NO含量在 14、6 0、90d均显著低于对照组 (P <0 0 5 )。染铅组皮质SOD活力在 30d后明显低于对照组 (P <0 0 5 ) ,高剂量组 30d时皮质SOD活力也较低剂量组低 (P <0 0 5 )。低剂量组皮质MDA含量在 30d后显著升高与对照组比 (P <0 0 5 ) ,高剂量组皮质MDA含量在 14d后显著升高 (P <0 0 5 ) ,且在 14d、90d时 ,高剂量组显著高于低剂量组 (P <0 0 5 )。提示铅所致神经毒性 ,可能与铅引起端脑皮质NO含量、NOS活力、SOD活力、MDA含量改变有关

Effects of lead on NO,NOS,SOD,MDA in rat cerebral cortex

To study the effects of lead exposure on nitric oxide (NO), nitric oxide synthase (NOS), super oxide dismutase (SOD) and malondialdehyde (MDA) in cerebral cortex of rats, Wistar rats are exposed to lead from drinking 0, 18.4, 184 mg/L lead acetate solution respectively. Results: Cerebral cortex NOS activity in low and high-dosed lead exposure groups are significantly increased at the time of 7 days (P<0.05), cerebral cortex NOS activity in low-dosed group is significantly decreased after 90 days and 30 days in high-dosed group (P<0.05) as compared with that of the controls. Cerebral cortex NO content is markedly decreased in low-dosed group after 90 days poisoning and 14 days , 60 days , 90 days in high-dosed group (P<0.05). SOD activity in low and high-dosed group is significantly decreased after 30 days exposure. MDA content is markedly increased after 14 days exposure in high-dosed group. Conclusion: It is suggested that the damage of cerebral cortex may caused by lead toxicity that results from the changes of NOS activity , NO level , SOD activity and MDA content in cerebral cortex .