深圳市某鞋厂H3亚型流行性感冒暴发流行病学调查

目的 调查分析深圳市某大型企业流行性感冒（流感）暴发的原因和特点以及流感实验室监测的结果，为流感暴发疫情的监测和控制提供科学依据。方法 对流感样病例（ILI）进行流行病学调查，采集患者咽拭子进行进行胶体金法初筛试验和荧光定量RT-PCR检测、流感病毒分离和鉴定。病毒分离采用鸡胚法和犬肾传代细胞（MDcK）法，病毒鉴定采用常量半加敏抑制试验（HI），抗体测定采用微量半加敏抑制试验。结果 ILI总罹患率为1．05％，以二分厂罹患率较高，女性罹患率远高于男性，发病年龄以15～岁组居多。从15份咽拭子样品中经荧光定量RT-PCR方法检出15份A型流感病毒核酸阳性，1份H3亚型流感病毒核酸阳性。培养分离鉴定出2株H3N2亚型流感病毒。经血凝抑制试验检测H3亚型流感抗体，9份急性期和恢复期血清（均来自同一患者）抗体滴度呈4倍以上增长。结论 该起暴发疫隋由H3N2亚型流感病毒引起，加强暴发疫情的病原学监测对控制痰隋有重要意义。

Epidemiological survey of H3 influenza outbreak in a shoe factory in Shenzhen

Objective The study was conducted to investigate the cause and characteristics of a influenza outbreak in a large-scale shoe factory in Shenzhen,and analyze the laboratory findings,provide the evidence for the surveillance and management of influenza outbreak.Methods Epidemiological survey was conducted for the influenza-like cases,and the throat swabs were taken from the patients for colloidal gold screening tests and quantitative fluorescent RT-PCR detection,and the isolation and identification of influen...