金雀异黄酮对去卵巢大鼠脑抗氧化作用的影响

目的研究金雀异黄酮对去卵巢大鼠大脑抗氧化作用的影响,为使用植物雌激素防治女性更年期后老年性痴呆提供依据。方法40只3月龄雌性SD大鼠,随机分为假手术组、去卵巢对照组、金雀异黄酮组和苯甲酸雌二醇组,给予相应手术和治疗。术后6周分析大鼠额叶、颞叶、海马和基底前脑的SOD和MDA值。结果假手术组颞叶和海马的SOD值低于基底前脑(P<0.05);额叶和颞叶的MDA值高于海马和基底前脑(P<0.05);去卵巢对照组基底前脑的SOD值显著低于假手术组、金雀异黄酮组和苯甲酸雌二醇组(P<0.05),MDA值显著高于假手术组、金雀异黄酮组和苯甲酸雌二醇组(P<0.05);假手术组、金雀异黄酮组及苯甲酸雌二醇组之间SOD值和MDA值差异无统计学意义(P>0.05)。结论去卵巢对照组大鼠基底前脑抗氧化能力显著降低,氧化损伤程度显著升高,金雀异黄酮替代治疗可增加去卵巢大鼠基底前脑的抗氧化能力,降低基底前脑的氧化损伤,可用于女性更年期后老年性痴呆的防治。

Investigation of genistein's antioxidation in the brain of ovariectomized

Objective To study antioxidative effects of genistein (Gs) in the brain of ovariectomized rats, and provide evidence to preventing or treating female postmenopausal Alzheimer's disease with phytoestrogen. Methods Forty female Sprague-Dawley rats, three month-old, were randomly assigned to four groups: sham-operated control (sham), ovariectomized control (OVX), genistin treated (Gs) and estradiol benzoate treated (EB) groups. Operations and treatments were performed respectively. Six weeks after operation, superoxide dismutase (SOD) and malondialdehyde (MDA) levels of frontal lobe, temporal lobe, hippocampus and basal forebrain were detected for statistical analysis. Results Temporal and hippocampal SOD levels of the sham group are lower than that of basal forebrain (P<0.05); frontal and temporal MDA levels are higher than those of hippocampus and basal forebrain (P<0.05). SOD level of OVX group is lower than those of sham, Gs and EB groups (P< 0.05). MDA value of OVX group is higher than those of sham, Gs and EB groups (P<0.05). But values of SOD and MDA among sham, Gs and EB groups have no significant difference. Conclusion Basal forebrain Antioxidative ability of OVX group decreased significantly, and oxidative damages increased remarkably. Gs replacement therapy can enhance basal forebrain antioxidative ability of OVX rats, and lower their oxidative damage, so can be used to preventing or treating female postmenopausal Alzheimer's disease.