大鼠低温海水淹溺肺脏血栓素B2及6-酮-前列腺素F1α的变化

目的观察大鼠低温海水淹溺肺脏血栓素B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)及T/P的变化规律,评价其对血气的影响. 方法将大鼠置于低温海水中游动,直至沉入水底濒临死亡,迅速取出测各组大鼠肛温,取心血行血气分析,并取各组及死亡组右肺组织,检测TXB2及6-Keto-PGF1α的含量,观察T/P变化;左肺组织计算干湿重比值(D/W). 结果 5 min组大鼠肛温(20.13±0.48)℃]、pH(6.68±0.03)、动脉血氧分压(PaO2)(45.00±6.30)mm Hg]、TXB2(97.46±17.46)ng/L]及6-Keto-PGF1α(25.59±8.12)ng/L]均降至最低点,与对照组比较,差异有显著性(P<0.01),二氧化碳分压(PaCO2)(89.18±5.10)mm Hg]升至最高点,与对照组比较,差异有显著性(P<0.01),上述指标随后均呈恢复趋势,但仅有240 min组、360 min组pH(7.31±0.05、7.32±0.07)及360 min组、死亡组TXB2(243.70±32.42)、(257.02±39.90)ng/L]接近正常对照组水平,且差异无显著性(P>0.05);T/P则呈上升趋势,360 min组T/P(10.92±3.84)升至最高点,与对照组比较,差异有显著性(P<0.01). 结论大鼠低温海水淹溺肺组织TXB2及6-Keto-PGF1α的含量受低温、低氧血症及酸中毒的影响,T/P失衡可能为影响血气指标改善的因素之一.

The changes of thromboxane B2(TXB2) and 6-keto-prostaglandin F1 alpha(6-Keto-PGF1α) in the lungs of rats drowned in hypothermic-sea-water

OBJECTIVE: To observe changes of thromboxane B2 (TXB2), 6-keto-prostaglandin F1 alpha(6-Keto-PGF1 alpha) and TXB2/6-Keto-PGF1 alpha (T/P) in lungs of rats drowned in hypothermic sea water and to assess their influence on the blood-gas. METHODS: Rats of different groups were drowned nearly to death in hypothermic sea water and then taken out of the water rapidly, observed at room temperature, after that the following steps were taken in 5, 15, 30, 60, 240 min and 360 min groups, that were 1 ml arterial blood taken from left heart for blood-gas analysis including pH, PaO2 and PaCO2, rectal temperature observed; at last, the ratio of left dry lungs with left wet lungs was assessed, TXB2 and 6-Keto-PGF1 alpha in right lungs were examined in all above groups and dead group(14 rats dead, only 4 examined). RESULTS: The rectal temperature(20.13 +/- 0.48) degree C], pH(6.68 +/- 0.03), PaO2(45.00 +/- 6.30) mm Hg)], TXB2(97.46 +/- 17.46) ng/L] and 6-Keto-PGF1 alpha(25.59 +/- 8.12) ng/L] dropped to the lowest point in the 5 minutes group(P < 0.01), while PaCO2(89.18 +/- 5.10) mm Hg] reached the highest point(P < 0.01), all above items from 5 minutes group then showed a recovering tendency, but only the pH in 240 minutes and 360 minutes groups as well as TXB2 in 360 minutes group and dead group reached near the level of normal control groups (P > 0.05); T/P had a rising tendency and reached the highest point in the 360 minutes group. CONCLUSIONS: The production and secretion of TXB2 and 6-Keto-PGF1 alpha were influenced by hypothermia, hypoxemia and acidosis, the imbalance of T/P could be one of factors influencing the improvement of blood gas index.