| HuR siRNA对肺腺癌A549 细胞增殖、迁移及侵袭的影响 |
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| 引用本文： | 申庆鹏,王俊,王宝成.HuR siRNA对肺腺癌A549 细胞增殖、迁移及侵袭的影响[J].中国肿瘤生物治疗杂志,2017,24(11):1271-1275. |
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| 作者姓名： | 申庆鹏 王俊 王宝成 |
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| 作者单位： | 1.济南军区总医院 肿瘤科，山东 济南 250031；2. 山东中医药大学 2015级硕士研究生班，山东济南250355,1.济南军区总医院 肿瘤科，山东 济南 250031；2. 山东中医药大学 2015级硕士研究生班，山东济南250355,1.济南军区总医院 肿瘤科，山东 济南 250031；2. 山东中医药大学 2015级硕士研究生班，山东济南250355 |
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| 基金项目： | 国家自然科学基金资助项目（No.81572875，81272619） |
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| 摘 要： | 目的：探讨HuR siRNA 对人肺腺癌A549 株细胞增殖、迁移及侵袭的影响及其可能机制。方法：HuR siRNA 瞬时转染肺腺癌A549 细胞24 h 后，CCK-8 实验、集落形成实验检测细胞的增殖能力，划痕实验检测细胞迁移能力，Transwell 实验检测细胞侵袭能力，qPCR和Western blotting 检测HuR及基质金属蛋白酶（MMP）-9 基因mRNA和蛋白质表达。结果：HuR siRNA能够抑制人肺腺癌A549 细胞的增殖、迁移及侵袭；HuR siRNA 显著降低A549 细胞HuR、MMP-9 mRNA和蛋白表达（P＜0.01）。结论：下调HuR能抑制肺腺癌A549细胞增殖、迁移及侵袭，其机制与下调MMP-9 有关。
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| 关 键 词： | 人抗原R 肺腺癌 肺腺癌A549 细胞 基质金属蛋白酶-9 侵袭 迁移 |
| 收稿时间： | 2017/6/11 0:00:00 |
| 修稿时间： | 2017/8/25 0:00:00 |
Effects of HuR siRNA on proliferation, migration and invasion of the lung adenocarcinoma A549 cells |
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| SHEN Qingpeng,WANG Jun and WANG Baocheng.Effects of HuR siRNA on proliferation, migration and invasion of the lung adenocarcinoma A549 cells[J].Chinese Journal of Cancer Biotherapy,2017,24(11):1271-1275. |
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| Authors: | SHEN Qingpeng WANG Jun and WANG Baocheng |
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| Institution: | 1. Department of Oncology, General Hospital of Jinan Military Command of PLA , Jinan 250031, Shandong,China; 2. 2015 Class for Master in Graduate School, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China,1. Department of Oncology, General Hospital of Jinan Military Command of PLA , Jinan 250031, Shandong,China; 2. 2015 Class for Master in Graduate School, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China and 1. Department of Oncology, General Hospital of Jinan Military Command of PLA , Jinan 250031, Shandong,China; 2. 2015 Class for Master in Graduate School, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China |
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| Abstract: | Objective: To explore effect of HuR siRNA on proliferation, migration and invasion of the human lung adenocarcinoma cell line A549 and its possible mechanism. Methods: After the A549 cells were transfected by HuR siRNA and cultured for 24 hours, CCK-8 assay and clone formation assay were used to detect proliferation ability of the A549 cells. Migration and invasion abilities of the A549 cells were respectively detected by scratch and Transwell assays. mRNA and protein expressions of HuR and matrix metalloproteinose-9 (MMP-9) were detected by RTqPCR and Western blotting assays. Results: HuR siRNA inhibited proliferation, migration and invasion of the human lung cancer cell line A549. HuR siRNA significantly reduced mRNA and protein expressions of HuR and MMP-9 in the A549 cells (P<0.01). Conclusion: Down-regulation of HuR could inhibit proliferation, migration and invasion of the lung adenocarcinoma A549 cells, and the inhibitions of migration and invasion of the A549 cells might be related to down-regulation of MMP-9. |
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| Keywords: | human antigen R (HuR) lung adenocarcinoma lung adenocarcinoma A549 cells MMP-9 invasion migration |
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