| The effect of interleukin-6 gene transfer on human cord blood megaka ryopoiesis |
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| 作者姓名： | " target="_blank">Yang Xingsheng Hitoshi Kurata" target="_blank">Hitoshi Kurata Kazuyuki Fujita" target="_blank">Kazuyuki Fujita Kenichi Tanaka" target="_blank">Kenichi Tanaka |
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| 作者单位： | Yang Xingsheng（Department of Obstetrics and
Gynecology, Qilu Hospital, Shandong University 250012 China）
Hitoshi Kurata（Department of Obstetrics and Gynecology, Niigata
University Graduate School of Medical and Dental Science,Japan）
Kazuyuki Fujita（Department of Obstetrics and Gynecology, Niigata
University Graduate School of Medical and Dental Science,Japan）
Kenichi Tanaka（Department of Obstetrics and Gynecology, Niigata
University Graduate School of Medical and Dental Science,Japan） |
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| 摘 要： | Chemotherapyremainsoneofthemosteffectivetreatmentsfordisseminatedneoplasia1 ] .However,theef ficacyofmanychemotherapeuticagentsislimitedbytheirtoxicitytonormaltissues,resultinginanarrowtherapeuticindex.Myelosuppressionisthemostfrequentacutedose limitingsideeffectresponsiblefordelaysindrugadminis trationordoseattenuationwithdecreasedantitumorre sponse2 4] .Particularly,thrombocytopeniaremainsasig nificantcauseofmorbidityincancerpatientsundergoingallogenicorautologousbonemarrow/bloodstemcellt…
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| 关 键 词： | 白细胞介素-6 作用机制 基因转染 造血干细胞 巨核细胞增多症 |
The effect of interleukin-6 gene transfer on human cord blood megakaryopoiesis |
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| Yang Xingsheng,Hitoshi Kurata,Kazuyuki Fujita,Kenichi Tanaka.The effect of interleukin-6 gene transfer on human cord blood megaka ryopoiesis[J].Current Advances In Obstetrics and Gynecology,2004,13(1):77-79. |
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| Authors: | Yang Xingsheng Hitoshi Kurata Kazuyuki Fujita Kenichi Tanaka |
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| Institution: | 1. Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University 250012 China
2. Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Science,Japan |
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| Abstract: | Objective:To investigate the effect of IL-6 gene transfer into human cord blood hematopoiefic stem cells on the production of megakaryocytic progenitors. Methods: IL-6 gene was transfected into human cord blood CD34 cells using a retrovirus vector with the aid of recombinant fibronectin fragments in the presence of a cocktail of cytokines ( SCF, IL-6, sIL-6R, FL, and TPO). Colony-forming units-megakaryocyte (CFU-MK) assays were performed as IL-6 gene transduced CD34 cells were incubated alone or in combination with IL-3 or sIL-6R, controlled with neoR gene transduced CD34 cells. Results: IL-6 alone or sIL-6R alone stimulated few CFU-MK colonies, the addition of sIL-6R to IL-6 gene transduced CD34 cells significantly enhanced the production of CFU-MK colonies. IL-6 gene transduced CD34 cells showed a modest synergistic effect with IL-3. Conclusion: These results suggest that IL-6 gene transfer may protect patients from chemotherapy-induced thrombocytopenia. |
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| Keywords: | Interleukin-6 Gene transfer Human cord bl ood Megakaryocytes |
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