2-甲氧雌二醇诱导人子宫内膜癌细胞株凋亡的实验研究

目的:探讨2-甲氧雌二醇(2-ME)对人子宫内膜癌细胞株KLE细胞凋亡的影响。方法:选用人子宫内膜癌细胞株KLE进行体外培养,实验组加入不同浓度2-ME,对照组不含2-ME。用电子显微镜(电镜)观察细胞形态变化;流式细胞仪(FCM)观察细胞的凋亡率及细胞周期的变化,以及免疫组织化学方法检测P53和Bcl-2的表达强度。结果:2-ME作用后G0/G1期细胞增加,并伴随G0/G1期细胞的增加,出现细胞凋亡峰和凋亡率的升高(P<0.05)。电镜下观察到KLE细胞染色体边集、核固缩、凋亡小体,P53和bc1-2阳性表达率明显下降。结论:2-ME对人子宫内膜癌KLE细胞株有诱导凋亡作用。

Experimental study on apoptosis in endometrial carcinoma cell induced by 2 -methoxyestradiol

Objective:To investigate the effect of 2-methoxyestradiol (2-ME) induction on apoptosis in human endometrial cell line KLE in vitro.Methods:Endometrial cancer cell line KLE originated from human endometrial adenocarcinoma was cultured in vitro and divided into study group (2-ME in different concentrations) and control group without 2-ME. Cell cycle and apoptotic percentage were detected by flow cytometry (FCM); morphological changes of apoptotic cells were observed by electronmicroscopy; immunohistochemical technique was used to determine the expression of p53 and bcl-2. Results:After treatment with 2-ME, the enhanced G0/G1 arrest was accompanied with the enhanced apoptotic peak and percentage, as well apoptotic cells were found more than those in control group.(P<0. 05) . By electron microscopy, there were many morphological characteristics of apoptosis including compaction and margination of nuclear chromosome and apoptotic bodies, and there was significant decrease in the expression of p53 and bcl-2 positive rate.Conclusion:2-ME could induce the apoptosis of KLE cells in vitro.