| PCR检测HBVDNA对HBsAg无症状携带者传染性的监测 |
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| 引用本文： | 秦立增,吴惠联,郭进林,岳玉娟,李焱,孙广莲.PCR检测HBVDNA对HBsAg无症状携带者传染性的监测[J].山东大学学报(医学版),1995(3). |
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| 作者姓名： | 秦立增 吴惠联 郭进林 岳玉娟 李焱 孙广莲 |
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| 作者单位： | 山东省医学科学院基础医学研究所 |
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| 摘 要： | 用多聚酶链反应和斑点杂交技术检测了５０例ＨＢｓＡｇ无症状携带者血清中ＨＢＶＤＮＡ，检出率分别为５０％（２５／５０）和５８％（２９／５０）。抗－ＨＢｃ、抗－ＨＢｅ和ＨＢｅＡｇ阳性者ＨＢＶＤＮＡ的检出率高于阴性者。单，ＮＨＢｓＡｇ阳性无症状携带者ＨＢＶＤＮＡ在血中呈不同程度地波动，其排毒情况无明显规律可循。
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| 关 键 词： | 乙型肝炎病毒 乙型肝炎表面抗原 脱氧核糖核酸 多聚酶链反应 |
POLYMERASE CHAIN REACTION FOR DETECTION OF HBV DNA TO SURVEY THE INFECTION OF HBsAg ASYMPOTOMATIC CARRIERS |
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| Qin Lizeng, Wu Huilian, Guo Jinlin, et al.POLYMERASE CHAIN REACTION FOR DETECTION OF HBV DNA TO SURVEY THE INFECTION OF HBsAg ASYMPOTOMATIC CARRIERS[J].Journal of Shandong University:Health Sciences,1995(3). |
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| Authors: | Qin Lizeng Wu Huilian Guo Jinlin |
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| Abstract: | Fifty serum samples of HBsAg asympotomatic carriers were detected by polymerase chain reaction (PCR) and dot hybridization respectively. The results showed that the HBV DNA positive rate was 50% to 58%.Its positive rate was much higher in the serum positive for anti-HBc, anti-HBe and HBeAg than the negative.The appearance of serum HBV DNA was fluctuation in the single HBsAg asympotomatic carriers proved no regularity for the elimination of hepatitis B virus. |
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| Keywords: | Hepatitis B surface antigens Hepatitis B virus DNA Polymerase chain reaction |
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