广西壮、汉族绝经后妇女维生素D受体基因型与骨密度的关系

目的 探讨维生素D受体基因(VDR)型在广西壮、汉族绝经后妇女中的分布及其与骨密度(BMD)的关系。方法 在广西居住20年以上、无血缘关系的健康绝经后妇女198名,其中三代均为壮族的116名,均为壮族的82名。记录他们的年龄、绝经年龄,测量他们的身高、体重。用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定受试者的VDR基因型;用双能X线吸收法测定髋部、腰椎和前臂的骨密度。结果 壮、汉两组妇女VDR基因型和VDR等位基因频率分布均无显著性差异(P>0.05);198名妇女BB、Bb、bb基因型检出率分别为6.57％、66.16％和27.27％;B、b等位基因分别为39.65％和60.35％。BB基因型组第二腰椎(L2)BMD较bb基因型组低10.03％(P=0.047),第四腰椎(L4)BMD分别较bb、Bb基因型组低9.63％(P=0.043)和12.44％(P=0.005)。BB基因型组骨质疏松发生率最高(46.15％),Bb基因型组次之(19.86％),bb基因型组最低(14.81％),差异有显著性(P=0.04)。结论 VDR基因型与广西壮、汉族绝经后妇女BMD有关联,BB基因型可能可作为预测广西壮、汉族绝经后妇女骨质疏松危险性的遗传学标志之一。

Relationship between vitamin D receptor genotypes and bone mineral density in Guangxi Zhuang and Han nationality postmenopausal women

Obsjective To investigate the distribution of vitamin D receptor(VDR) genotypes and the as-sociation of VDR genotypes with bone mineral density (BMD) in Guangxi Zhuang and Han nationality post-menopausal women. Methods 198 postmenopausal women who have resided in Guangxi over 20 years,and their grandparents and maternal grandparents were of the same nationalityies were included in this study. Their age, age at natural menopause height, and weight were recorded.Their VDR genotypes were idetermined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the Bsm I enzyme; their BMD was deter-mined by dual energy X-ray absorptiometry(DEXA) at proximal femur, lumbar vertebrae (L2-L4) and radius. Results The distribution of the VDR genotypes did not differ significantly between Zhuang and Han nationalities ( P > 0.05 ) . Within the tested samples, there were 6.57 % BB homozygotes, 66.16 % Bb hterozygotes, and 27.27% bb homozygotes. The BMD of L2 in the BB group was 10.03% lower than thatin bb group ( P = 0.047) ; the BMD of L4 in the BB group was 9.63% and 12.44% lower than those in bb and Bb group, respectively (P = 0.043 and P = 0.005, respectively). The osteoporosis prevalence rates in BB, Bb and bb women were 46.15% , 19.85% and 14.81% , respectively, with a significant difference(P = 0.04) .Conclusion VDR genotypes are associated with vertebral BMD and osteoporosis in Guangxi Zhuang and Han nationality postmenopausal women, and BB genotype may serve as a hereditary predirector of osteoporosis in them.