牙龈卟啉单胞菌对人脐静脉内皮细胞一氧化氮生成的影响

目的 观察牙龈卟啉单胞茵(Porphyromonas gingivalis,Pg)对人脐静脉内皮细胞皮细胞功能损伤的途径,以期为牙周病在动脉粥样硬化发病机制中的作用提供依据.方法 应用厌氧罐培养Pg ATCC33277,用感染复数(multiplicity of infection,MOI)1:10、1:100、1:1000的Pg干预HUVEC,未受Pg干预的HUVEC作为阴性对照组,分别于4、8、12、24 h时收集细胞上清液,利用硝酸还原酶法测定细胞上清液中NO的浓度.结果 在24 h内,Pg MOI 1:10、1:100均能促进HUVEC NO的生成,与阴性对照组相比差异有统计学意义(P＜0.05);Pg MOI 1:1000干预HUVEC后,12 h内可促进HUVEC NO的生成,但与阴性对照组相比差异无统计学意义,24 h时HUVEC NO的生成降低(57.83±9.09)mmol/L],与其他各组相比差异均有统计学意义(P＜0.05).结论 Pg对内皮细胞NO的生成有影响.Pg MOI 1:10、1:100能够促进内皮细胞NO的生成,Pg MOI 1:1000则抑制内皮细胞NO的生成.

Effect of Porphyromonas gingivalis on nitric oxide in cultured human umbilical vein endothelial cells

Objective To observe the effect of Porphyromonas gingivalis(Pg)on the production of nitric oxide(NO)in cultured human umbilical vein endothelial cells(HUVEC).and to investigate the pathway of damaging endothelial function by Pg.Methods Pg ATCC33277 was cultured in anaerobic jar,and HUVEC was treated with various concentrations of Pg ATCC33277 at multiplicity of infection(MOI)of 1:10,1:100 and 1:1000 for 4,8,12,24 h respectively.The cells supernatants were collected and stored at-70℃and NO concentration in the cells supematanta was measured by nitrate reductase assay.Results Within 24 h,Pg at MOI of 1:10 and 1:100 stimulated the release of nitric oxide in cultured HUVEC.Within 12 h,Pg at an MOI of 1:1000 group increased NO production,and NO decreased at 24 h.Conclusions Pg has an effect on the production of NO.Low concentrations of Pg stimulated release of nitric oxide in endothelial cells but high concentrations can decrease the release of NO.