电针内关、大肠俞对IBS模型大鼠行为及结肠Cajal间质细胞的影响

目的 观察电针内关和大肠俞穴对肠易激综合征模型大鼠内脏敏感性、情绪心理行为及结肠Cajal间质细胞(ICC)的变化,探讨针刺不同经穴治疗肠易激综合征的疗效及部分作用机制.方法 选取Wistar大鼠制备肠易激综合征模型,随机分为:空白对照组、模型对照组、电针内关组、电针大肠俞组(简称治疗组),造模成功后,治疗组进行电针处理.治疗结束后,采用腹部回撤反射对大鼠进行内脏敏感性评估,观察潜伏期及收缩波个数;采用旷场实验测试大鼠情绪心理,记录大鼠水平和垂直活动次数;采用免疫组织化学法检测结肠ICC的c-kit阳性表达.结果 1)内脏敏感性评估:与空白对照组比较,模型对照组潜伏期明显缩短,收缩波个数明显增多(P<0.05,P<0.01);与模型对照组比较,治疗组潜伏期延长,收缩波个数明显减少(P<0.05,P<0.01);2)旷场实验:与空白对照组比较,模型对照组水平与垂直活动次数减少(P<0.01,P<0.05);与模型对照组比较,治疗组水平活动次数增多(P<0.05),且电针内关组的治疗效果更佳;3)结肠ICC特异性标志物c-kit表达水平:与空白对照组比较,模型对照组结肠c-kit的阳性表达水平明显增强(P<0.01);与模型对照组比较,治疗组结肠c-kit的阳性表达水平明显减弱(P<0.01).结论 电针内关和大肠俞穴均可降低IBS模型大鼠的内脏敏感性,并能改善大鼠情绪心理行为的变化,其作用可能与其调节结肠ICC有关.

Effect of electroacupuncture Neiguan and Dachangshu points on IBS model rats behavior and the colonic interstitial cells of Cajal level

Objective To observe electroacupuncture Neiguan and Dachangshu acupoint on irritable bowel syndrome (IBS) model rats with visceral sensitivity, psychology behavior and changes of interstitial cells of Cajal (ICC) in colon and to investigate the effects of acupuncture on different acupoints treating irritable bowel syndrome difference in the efficacy and mechanism.Methods Wistar rats of IBS model were randomly divided into: blank control group, model control group, EA Neiguan group,EA Dachangshu group (the treatment group). After successful modeling, the treatment group was treated with Electroacupuncture.After the end of treatment with Abdominal withdrawal reflex, evaluation of visceral sensitivity in rats and observe the latency and the contraction wave number; By open field test in rats the psychological, rat horizontal and vertical activity times record; Using immunohistochemical method to detect the colon of c-kit positive expression.Results 1) Evaluation of visceral sensitivity: compared with the blank control group, model control group was shortened, the contraction wave number increased (P<0.05,P<0.01); Compared with the model group, the treatment group prolonged latency of a contraction wave significantly reduced the number (P<0.05, P< 0.01); 2) Open field test: compared with blank control group, model control group, the level of vertical activity and reduce the number of activities (P<0.01,P<0.05); Compared with the model group, the treatment group level number increase (P<0.05), And the effect of EA Neiguan group was better.3) Colon c-kit expression: compared with the blank control group, model group of colon c-kit positive expression was increased (P<0.01); Compared with the model group, the treatment group of colon c-kit positive expression was decreased (P<0.01).Conclusion Electroacupuncture"Neiguan" and "Dachangshu"can reduce the visceral sensitivity of IBS model rats, and can improve the changes of emotional and psychological behavior in rats, which may be related to the regulation of ICC in the colon.