3种消毒剂用于内镜洗消机消毒效果的研究

为了解3种消毒剂用于全自动胃镜洗消机消毒效果,在实验室进行了定量杀菌试验和模拟现场试验。结果,采用水冲洗3min,超声雾化消毒剂气雾熏蒸不同时间,然后再用水冲洗程序处理。用200mg/L二氧化氯、20g/L戊二醛气雾熏蒸2min,对置于内镜截物网4角及中央部位钢片上金黄色葡萄球菌清除率达100%;含有效氯160mg/L的酸性氧化电位水作用2min,清除率为99.98%;对枯草杆菌黑色变种芽孢清除率只有二氧化氯能达到99.9%以上。模拟现场试验用同样方法处理,3种消毒剂对内镜表面和内腔污染细菌清除率最高为75.26%,最低为32.72%。结论,3种消毒剂在水冲洗情况下,雾化熏蒸在实验室条件下能达到消毒合格,模拟现场条件下达不到消毒要求。

STUDY ON DISINFECTION EFFICACY OF THREE DISINFECTANTS USED IN ENDOSCOPE WASHING-DISINFECTION MACHINE

In order to know the disinfection efficacy of 3 disinfectants used in full-automatic gastroscope washing-disinfection machine, quantitative germicidal test in laboratory and simulated field trial were carried out. Results: The treatment procedures used were washing with water for 3 min, aerosol fumigation with ultrasonic aerosolized disinfectant for different periods of time and then washing with water again. Fumigation with aerosol of 200 mg/L chlorine dioxide and 20 g/L glutaraldehyde for 2 min eradicated 100% of Staphylococcus aureus on steel coupons put at 4 corners and central part of carrier net of the endoscope. Electrolyzed oxidizing water containing available chlorine 160 mg/L with a 2 min contact time eradicated 99.98% of the bacteria. For eradication of spores of Bacillus subtilis var. niger, only chlorine dioxide could attain eradication rate of higher than 99.9%. Same methods were used for treatment in simulated field trial. The eradication rate of the bacteria contaminating the surface and internal cavities of the endoscopes by 3 disinfectants was at maximum 75.26% and at minimum 32.72%. Conclusion: Under laboratory conditions and combined with water washing, fumigation with aerosols of the 3 disinfectants can attain eligible disinfection, while in simulated field conditions, it can not fulfill the requirement of disinfection.