| 依托咪酯对大鼠肠缺血-再灌注致视网膜损伤的保护作用 |
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| 引用本文： | 赵娟,顾恩华,张抗抗. 依托咪酯对大鼠肠缺血-再灌注致视网膜损伤的保护作用[J]. 临床麻醉学杂志, 2011, 27(3) |
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| 作者姓名： | 赵娟 顾恩华 张抗抗 |
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| 作者单位： | 天津市眼科医院麻醉科,天津医科大学眼科临床学院,300020 |
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| 摘 要： | 目的 探讨依托咪酯是否可对大鼠肠缺血-再灌注引起的视网膜损伤产生保护作用.方法 Wistar大鼠24只分为三组:假手术组(S组)、缺血-再灌注组(Ⅰ组)、依托咪酯组(E组).S组仅分离肠系膜上动脉(SMA),不夹闭;Ⅰ组夹闭SMA 1 h,再灌注2 h,下腔静脉持续输注生理盐水10ml·kg<'-1>·h<'-1>;E组则输注依托咪酯0.2 mg·kg<'-1>·h<'-1>.静脉采血2 ml,测定血清肌酐(Cr)水平.剥离视网膜,测定其组织匀浆中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量.病理切片观察小肠和视网膜病理变化.结果 与S组比较,Ⅰ组SOD活性显著降低,MDA含量和Cr水平显著升高(P<0.05);与Ⅰ组比较,E组SOD活性的增高和MDA含量与Cr水平降低(P<0.05).病理观察:与S组比较,Ⅰ组肠上皮下间隙扩张,黏膜上皮层与固有层中度分离,中性粒细胞增多,部分绒毛顶端破损.视网膜内层明显水肿,神经节细胞分散,排列不规则;外核层分解、破坏.E组小肠组织结构轻度改变,部分绒毛顶端破损.视网膜内层水肿明显减轻,末梢小血管开放,细胞排列较规则.结论 依托咪酯对大鼠小肠缺血-再灌注所致的视网膜损伤具有保护作用.
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| 关 键 词： | 依托眯酯 缺血-再灌注损伤 视网膜 大鼠 |
Protection effect of etomidate on the retina injury induced by intestinal ischemia-reperfusion in rats |
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| ZHAO Juan,GU En-hua,ZHANG Kang-kang. Protection effect of etomidate on the retina injury induced by intestinal ischemia-reperfusion in rats[J]. The Journal of Clinical Anesthesiology, 2011, 27(3) |
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| Authors: | ZHAO Juan GU En-hua ZHANG Kang-kang |
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| Affiliation: | ZHAO Juan,GU En-hua,ZHANG Kang-kang.Department of Anesthesiology,Tianjin Eye Hospital,Tianjin Medical University,Clinical College of Ophthalmology,Tianjin 300020,China |
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| Abstract: | Objective To investigate the protection effect of etomidate on the retina injury induced by intestinal ischemia-reperfusion in rats.Methods Twenty-four adult male wistar rats were randomly divided into three groups:sham-operated group(group S),ischemia-reperfusion group(group I),etomidate group(group E).Superior mesenteric artery(SMA)was separated from abdominal cavity in group S,SMA were clamped for 60 min with a clip followed by 120 min reperfusion in groups I and E,0.9% sodium chloride were injected cont... |
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| Keywords: | Etomidate Ischemia-reperfusion injury Retina Rats |
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