| ATP敏感性钾通道在七氟醚预处理延迟相减轻大鼠心肌缺血-再灌注损伤中的作用 |
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| 引用本文： | 肖艳英,常业恬,冉珂,李双凤. ATP敏感性钾通道在七氟醚预处理延迟相减轻大鼠心肌缺血-再灌注损伤中的作用[J]. 临床麻醉学杂志, 2011, 27(3) |
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| 作者姓名： | 肖艳英 常业恬 冉珂 李双凤 |
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| 作者单位： | 中南大学湘雅二医院麻醉科,长沙市,410011 |
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| 摘 要： | 目的 探讨线粒体ATP敏感性钾通道(mito-KAar)在七氟醚预处理延迟相减轻大鼠心肌缺血-再灌注(I-R)损伤中的作用.方法 雄性SD大鼠80只随机均分为五组:假手术组(A组);I-R组(B组),左冠状动脉前降支结扎30 min后再灌注120 min;七氟醚预处理组(C组),I-R前24 h吸人2.5%七氟醚1 h;七氟醚预处理+mito-K<,ATP>抑制剂5-羟基癸酸(5-HD)组(D组),七氟醚预处理前尾静脉注射5-HD 5 mg/kg;单纯5-HD组(E组).再灌注120 min后各组取10只大鼠测定心肌缺血危险面积与梗死面积,酶联免疫吸附(ELISA)法检测血清肌钙蛋白I(cTnI)浓度,各组另取6只大鼠采用免疫印迹检测左室心肌Bc1-2及Bax蛋白表达.结果 七氟醚预处理可减少I-R引起的心肌梗死面积、降低血清cTnI水平,上调心肌Bc1-2表达、下调Bax表达(P<0.05).而这种效应可以被5-HD所抑制.结论 七氟醚预处理延迟相可减轻大鼠心肌I-R损伤,可能与mito-K<,ATP>开放引起的Bc1-2及Bax表达变化有关.
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| 关 键 词： | 七氟醚 心肌再灌注损伤 缺血预处理 ATP敏感性钾通道 凋亡调控蛋白 |
Role of mitochondrial ATP sensitive potassium channel in delayed preconditioning with sevoflurane reducing myocardial ischemia-reperfusion injury in rats |
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| XIAO Yan-ying,CHANG Ye-tian,RAN Ke,et al.. Role of mitochondrial ATP sensitive potassium channel in delayed preconditioning with sevoflurane reducing myocardial ischemia-reperfusion injury in rats[J]. The Journal of Clinical Anesthesiology, 2011, 27(3) |
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| Authors: | XIAO Yan-ying CHANG Ye-tian RAN Ke et al. |
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| Affiliation: | XIAO Yan-ying,CHANG Ye-tian,RAN Ke,et al.Department of Anesthesiology,Xiangya Second Hospital,Center South University,Changsha 410011,China |
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| Abstract: | Objective To investigate the role of mitochondrial ATP sensitive potassium channel(mito-KATP)in the delayed cardioprotection produced by sevoflurane in ischemia-reperfusion(I-R)injured rats.Methods Eighty male Sprague-Dawley rats were randomly divided into five groups:a sham operation group(group A);an ischemia-reperfusion injury group(group B),occlusion of left anterior descending coronary artery for 30 min and followed by 120min of reperfusion;a sevoflurane preconditioning group(group C),breathing 2.5% se... |
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| Keywords: | Sevoflurane Myocardial reperfusion injury Ischemia preconditioning Mitochondrial ATP sensitive potassium channel Apoptotic protein |
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