| 神经营养因子-3在颞叶癫(癎)后的表达与海马苔藓纤维发芽的关系 |
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| 引用本文： | 梁霁,郑金瓯,余璐,陈子蓉,邓晓清.神经营养因子-3在颞叶癫(癎)后的表达与海马苔藓纤维发芽的关系[J].临床神经病学杂志,2011,24(1). |
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| 作者姓名： | 梁霁 郑金瓯 余璐 陈子蓉 邓晓清 |
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| 作者单位： | 广西医科大学第一附属医院神经内科,530021 |
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| 摘 要： | 目的 探讨神经营养因子-3(NT-3)在颞叶癫(癎)大鼠海马内表达与苔藓纤维发芽(MFS)的可能关系.方法 大鼠侧脑室注射红藻氨酸(KA)建寺颞叶癫(癎)模型后,侧脑室注射NT-3反义寡核苷酸(ASODN)及正义寡核苷酸(SODN);应用免疫组化法观察海马NT-3表达;应用Timm银染方法,光镜和电镜观察海马MFS,并与空白对照组及脂质体对照组进行比较.结果 致(癎)后大鼠海马齿状回NT-3表达下降;海马苔藓纤维明显粗乱,侧支发芽;齿状回内分子层可见银标记突触末端,主要为轴-树型非对称突触,其中ASODN组海马NT-3蛋白水平降低及MFS程度更明显.结论 KA致痢和NT-3 ASODN均能降低大鼠海马齿状回NT-3表达,增加MFS程度.提示NT-3可能通过对MFS及突触重组作用来影响颞叶癫(癎)的发生和发展.
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| 关 键 词： | 神经营养因子-3 癫(癎) 反义寡核苷酸 苔藓纤维发芽 突触 |
Correlation between neurotrophin-3 and mossy fibers sprouting hi hippocampus after temporal epilepsy |
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| LIANG Ji,ZHENG Jin-ou,YU Lu,et al..Correlation between neurotrophin-3 and mossy fibers sprouting hi hippocampus after temporal epilepsy[J].Journal of Clinical Neurology,2011,24(1). |
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| Authors: | LIANG Ji ZHENG Jin-ou YU Lu |
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| Institution: | LIANG Ji,ZHENG Jin-ou,YU Lu,et al.Department of Neurology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China |
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| Abstract: | Objective To investigate the potential relation of abnormal neural circuits and express changes of neurotrophin-3(NT-3) in hippocampus of experimental temporal epilepsy rats.Methods Built experimental temporal epilepsy models of rats by injecting kainic acid(KA) in lateral ventricle of adult rats,injected NT-3 antisense oligonucleotide(ASODN) and sense oligonucleotide(SODN),then measure the expression of NT-3 in hippocampus of rats by immunohistochemistry.Mossy fiber sprouting(MSF) and synapse restitution i... |
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| Keywords: | neurotrophin-3 epilepsy antisense oligonucleotide mossy fiber sprout synapse |
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