前列地尔脂质体对血小板功能的影响及注射部位的刺激性的实验研究

目的:观察静脉注射前列地尔脂质体对血小板功能的影响以及对静脉的刺激性,并与注射用前列地尔比较。方法:用玻球法测定SD大鼠血小板粘附率,放射免疫法测定血浆凝血恶烷B_2(TXB_2)以及6-酮- 前列腺素F_(1α)(6-Keto-PGF_(1α)),昆明种小鼠剪尾测定出血时间,测定二磷酸腺苷(ADP)诱导呼吸喘促时间,并用大耳白兔耳缘静脉注射比较刺激性。结果:前列地尔脂质体可以显著降低大鼠血小板粘附率,同时血浆中 TXB_2的浓度降低,6-Keto-PGF_(1α)的浓度升高,并可延长小鼠出血时间,缩短注射ADP后的呼吸喘促时间,与对照组比较P<0．05,与注射用前列地尔比较P>0．05。此外,对注射局部的刺激性比注射用前列地尔少。结论:前列地尔脂质体对血小板功能的影响与注射用前列地尔相当,但刺激性大大降低。

Experimental study of effect of alprostadil liposomes on platelet function and local site of irritation

AIM: To evaluate the effect of alprostadil liposomes on platelet function and its irritation with vein ; and comparison to alprostadil for injection. METHODS: The platelet adhesiveness in SD rats were assayed by rotating glass—globe method. The concentration of TXB_2 and 6-Keto-PGF_(1α) in blood plasma was assayed by radioimmunoassay. The tail bleeding time was taken by shearing tail method,and the time of respiratory distress due to pulmonary embolism in mice induced by ADP were also recored. The local site irritation induced by alprostadil liposomes was compared with that of alprostadil for injection through rabbit ear marginal vein injection. RESULTS: Alprostadil liposomes could significantly decrease platelet adhesiveness rate in rats and prolong the time of relieved respiratory distress in mice together with decrease of concentration TXB_2 and increase of 6-Keto-PGF_(1α) in blood plasma of rats which were significantly superior to those of control (P < 0.05). Alprostadil liposomes could also prolong the tail bleeding time in mice and significantly decrease the intravenous irritation in rabbits. CONCLUSION: The effect of alprostadil liposomes on platelet function is equivalent to alprostadil for injection,with conspicuous low irritation.