Mithramycin A对肝癌细胞Huh-7增殖的影响

目的研究抗肿瘤抗生素光辉霉素（Mithramycin A,MIT）对人肝癌细胞Huh-7的作用。方法CCK-8比色法观察MIT对Huh-7细胞的生长抑制作用,Hoechst 33342/PI双荧光染色分析细胞凋亡,流式细胞仪检测肿瘤细胞凋亡率。结果MIT可抑制Huh-7细胞的生长,半数抑制浓度（IC50）为60.12 nmol.L^-1。荧光显微镜观察可见细胞凋亡特征性改变;浓度为5×10^-2μmol.L^-1的MIT作用72 h后,可明显诱导Huh-7细胞凋亡,流式细胞仪检测肿瘤细胞出现典型亚二倍体凋亡小峰。结论MIT对Huh-7细胞有明显的诱导凋亡和生长抑制作用,具有浓度、时间依赖性。

The effect of Mithramycin A on proliferation of human liver carcinoma cell line Huh-7

Objective To examine the effect of Mithramycin A （MIT） on the human liver tumour cell line Huh-7. Methods Huh-7 cells were treated with MIT at different concentrations, and the inhibitory effect on cell growth was analyzed by CCK-8 colorimetric assay and the cells were observed under fluorescent microscope for cell apoptosis by using Hoechst33342/PI double fluorescent staining approach. The effect of MIT on the apoptotic rate of tumour cells was analyzed by flow cytometry. Results The treatment of Huh-7 cells with MIT inhibited cell growth and IC50 value of MIT for Huh-7 cells was 60. 12 μmol.L^-1. The characteristic signs of apoptosis were observed under a fluorescent microscope, and apoptosis of Huh-7 cells can be induced by MIT at concentration of 5 × 10^-2μmol.L^- 1 in 72 h. After 72 h treatment with MIT, some apoptotic cells were detected and a typical subdiploid peak was observed by flow cytometry. Conclusion MIT can efficiently induce apoptosis of Huh-7 cells and inhibit the cell growth in a dose-dependent and timedependent manet