| 何首乌蒽醌苷对小鼠细胞免疫功能的影响 |
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| 引用本文： | 孙桂波,郭宝江,李续娥,黄俊柅,徐惠波,孙晓波.何首乌蒽醌苷对小鼠细胞免疫功能的影响[J].中药药理与临床,2006,22(6):30-32. |
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| 作者姓名： | 孙桂波 郭宝江 李续娥 黄俊柅 徐惠波 孙晓波 |
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| 作者单位： | 1. 广东省植物发育生物工程重点实验室,华南师范大学生命科学学院,广州,510631
2. 吉林省中医中药研究院,长春,130021 |
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| 基金项目： | 广东省广州市科技局科研项目;广东省植物发育生物工程重点实验室开放基金 |
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| 摘 要： | 目的:研究何首乌蒽醌苷(AGPMT)体外对小鼠细胞免疫功能的影响。方法:将小鼠淋巴细胞或巨噬细胞与不同浓度的AGPMT共培养,用XTT法测T、B淋巴细胞的增殖能力、对MLR的影响及对MitC所致淋巴细胞增殖抑制的拮抗作用;用中性红染色法观察AGPMT对巨噬细胞吞噬功能、NK细胞活性、小鼠脾细胞分泌TNF活性的影响。结果:AGPMT在体外可明显促进小鼠T、B淋巴细胞的增殖,增强巨噬细胞吞噬中性红的能力,提高NK细胞活性及分泌TNF活性,并可促进MLR,拮抗MitC所致淋巴细胞增殖的抑制作用。结论:AGPMT具有免疫增强作用。
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| 关 键 词： | 何首乌蒽醌苷 淋巴细胞 巨噬细胞 细胞免疫 |
The effect of anthraquinone glycoside from Polygonum multiflorum Thunb on cellular immunological function in mice |
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| Sun Guibo,Guo Baojiang,Li Xueer,Huang Junni,Xue Huebao,Sun Xiaobao.The effect of anthraquinone glycoside from Polygonum multiflorum Thunb on cellular immunological function in mice[J].Pharmacology and Clinics of Chinese Materia Medica,2006,22(6):30-32. |
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| Authors: | Sun Guibo Guo Baojiang Li Xueer Huang Junni Xue Huebao Sun Xiaobao |
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| Institution: | 1.Guangdong Key Lab of Biotechnology for Plant Development ,South China Normal University, Guangzhou 510631;2.Academy of Traditional Chinese Medicines and Mgteria Medica of Jilin Province. Changchun 130021 |
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| Abstract: | Objective:To study the effect of anthraquinone glycoside from Polygonum multiflorum Thunb.(AGPMT) in vitro on cellular immunological function in mice. Methods:The mouse lymphocytes or macrophages were co-cultured with AGPMT.T and B lymphocyte proliferation,mixed lymphocyte reaction(MLR) and the antagonistic effect on reduced lymphocyte proliferation by mitomycin(Mit C) was determined by XTT method. Macrophages phagocytosis,tumor necrosis factor (TNF) secretion activity of mouse spleen cells and activity of natural killer(NK) cells was determined by using neutral red assay. Results: AGPMT could significantly accelerate T and B lymphocytes proliferation in vitro,improve macrophages phagocytosis , increase TNF secretion activity and activity of NK cells, accelerate mixed lymphocyte reaction, antagonize restraining effect of lymphocyte proliferation by Mit C. Conclusion: AGPMT could potentiate the immunological function. |
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| Keywords: | anthraquinone glycoside from Polygonum multiflorum Thunb lymphocyte macrophage cellular immunological function |
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