Pathogenic activity of circulating anti-desmoglein-3 autoantibodies isolated from pemphigus vulgaris patients |
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| Authors: | Joanna Narbutt Joanna Boncela Katarzyna Smolarczyk Cezary Kowalewski Katarzyna Wozniak Jolanta Dorota Torzecka Anna Sysa-Jedrzejowska Czes?aw S. Cierniewski Aleksandra Lesiak |
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| Affiliation: | 1.Department of Dermatology, Medical University of Lodz, Poland;2.Centre of Medical Biology, Polish Academy of Sciences, Lodz, Poland;3.Department of Dermatology, Medical University of Warsaw, Poland |
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| Abstract: | IntroductionThere are scarce data on immunochemical properties of pemphigus antibodies detected in clinical remission in pemphigus vulgaris (PV) patients. The aim of the study was to compare biological activity of anti-Dsg3 autoantibodies purified from the sera of PV patients in active stage and in clinical remission.Material and methodsThe effect of purified antibodies on expression of procaspase-3, Bax, Bcl-2, uPAR, IL-1β, IL-6, and TNF-α mRNAs in the HaCaT keratinocytes was evaluated by Western blot and RT-PCR method.ResultsIncubation of HaCaT cells with anti-Dsg-3 autoantibodies caused their binding to cell membranes surfaces. Anti-Dsg3 autoantibodies isolated from the patients in active stage and clinical remission showed proapoptotic effect, caused enhanced expression of analyzed proinflammatory cytokines’ mRNAs and uPAR mRNA.ConclusionsOur data revealed similar pathogenic activity of anti Dsg-3 autoantibodies isolated from active and clinical remission PV patients. |
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| Keywords: | pemphigus vulgaris anti-Dsg-3 antibodies acantholysis keratinocyte culture apoptosis |
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