顺铂干预小鼠Lewis肺癌的MMP-9、TIMP-1表达及其相关性

背景与目的：肺癌转移是疾病进展和治疗失败的主要原因，基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）、组织金属蛋白酶抑制物-1（tissue inhibitor of metalloproteinase-1，TIMP-1）作为调控细胞外基质降解的蛋白与肺癌转移关系密切。本研究观察顺铂对Lewis肺癌MMP-9和TIMP-l表达的影响，并探讨两者的关系和在转移中的作用。方法：建立Lewis肺癌C57BL／6小鼠肿瘤模型，顺铂组予以腹腔内注射顺铂溶液，以正常小鼠和荷瘤小鼠作对照。采用ELISA法和免疫组化法检测MMP-9、TIMP-1在血清和瘤体中的表达。结果：顺铂抑瘤率41．2％，抑制转移率39．0％。荷瘤小鼠瘤体中MMP-9、TIMP-1阳性率均为100％，血清浓度均高于正常对照组（P〈0．05）；顺铂组的血清MMP-9、TIMP-1浓度和瘤体MMP-9阳性率均低于荷瘤对照组（P〈0．05）。血清及瘤体的MMP-9表达、瘤体TIMP-1表达均与瘤重、肺转移数成正相关（P〈0．05）。血清及瘤体的MMP-9表达与TIMP-1表达均成正相关（r=0．617和0．695，P〈0．05）；血清MMP-9／TIMP-1的比值趋向于一个常量，其数值呈正态分布，均数为1．72。结论：Lewis肺癌中MMP-9和TIMP-1均呈高表达，两者与癌细胞的浸润和转移均有关；顺铂对血清MMP-9、TIMP-1浓度和瘤体MMP-9表达的下调作用是其抗转移作用的机理之一。

Effect of cisplatin on expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 and their correlations in Lewis lung cancer in mice

Background and Objective: Metastasis of lung cancer is the leading cause of disease progression and treatment failure. Matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) are related to the metastasis of lung cancer via regulating the degradation of extracellular matrix. This study was to observe the impacts of cisplatin (DDP) on the expression of MMP-9 and TIMP-1 in Lewis lung cancer, and explore their correlations and roles in metastasis. Methods: Lewis lung cancer mode...